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Expression and promoter activity of endogenous retroviruses in the Olive flounder (Paralichthys olivaceus)

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Abstract

Olive flounder (Paralichthys olivaceus) is regarded as one of economical fish species in the world. Genome information of Olive flounder has been revealed by next generation sequencing. Endogenous retroviruses (ERVs) as a member of transposable elements have been involved in functional roles of various genes in variety organisms. In this study, we identified an Olive flounder ERV (OF-ERV5) using RepeatMasker program, and examined expression pattern of pol gene of OF-ERV5, which indicated the high expression in two kidney samples (head kidney and body kidney). In addition, the 5′LTR sequences of OF-ERV5 are cloned into pGL4.11 vectors to confirm promoter activity. Luciferase assay indicated that the OF-ERV5_LTR showed promoter activity in both HepG2 and HINAE cell lines. These data could be of great use for further study to understand biological function of transposable elements in Oliver flounder.

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Acknowledgments

This research was supported by a grant from Marine Biotechnology Program (PJT200620, Genome Analysis of Marine Organisms and Development of Functional Application) Funded by Ministry of Oceans and Fisheries, Korea.

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Correspondence to Won Kim or Heui-Soo Kim.

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Pusan National University guidelines for the care and use of animals were followed.

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13258_2016_404_MOESM1_ESM.pptx

Supplementary material 1 RT-PCR analysis of mRNA for the expression of 18S rRNA gene in six samples for normalization. (PPTX 114 kb)

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Nam, GH., Gim, JA., Lee, HE. et al. Expression and promoter activity of endogenous retroviruses in the Olive flounder (Paralichthys olivaceus). Genes Genom 38, 539–545 (2016). https://doi.org/10.1007/s13258-016-0404-8

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