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Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.)

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An Erratum to this article was published on 11 May 2016

Abstract

The cultivated peanut is important oil crop and salt stress seriously influences its development and yield. Tolerant varieties produced using transgenic techniques can effectively increase peanut plantation area and enhance its yields. However, little is known about how gene expression is regulated by salt stress in peanut. In this study, we screened genes regulated by salt stress in peanut roots using microarray technique. In total, 4828 up-regulated and 3752 down-regulated probe sets were successfully identified in peanut roots subjected to 3 and 48 h of salt stress. Data analysis revealed that different response groups existed between the up and down-regulated probe sets. The main up-regulated biological processes involved in salt stress responses included transcription regulation, stress response, and metabolism and biosynthetic processes. The main down-regulated biological processes included transport processes, photosynthesis and development. The Kyoto encyclopedia of genes and genomes pathway analysis indicated that metabolic pathway, biosynthesis of unsaturated fatty acids and plant–pathogen interaction, were mainly up-regulated in peanut under salt stress. However, photosynthesis and phenylalanine metabolism were mainly down-regulated during salt stress. The function of some probe sets in salt stress regulation was not clarified (e.g., protein functioning in cell cycle regulation and xylem development). Many of the genes we identified lacked functional annotations and their roles in response to salt stress are yet to be elucidated. These results identified some candidate genes as potential markers and showed an overview of the transcription map, which may yield some useful insights into salt-mediated signal transduction pathways in peanut.

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Acknowledgments

This study was supported by Grants from China Agriculture Research System (CARS-14), the National Natural Science Foundation of China (31000728; 31200211), the Natural Science Fund of Shandong Province (ZR2011CQ036, ZR2012CQ031, ZR2014YL011, ZR2014YL012), the Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences (the Youth Scientific Research Foundation of Shandong Academy of Agricultural Sciences); the Fund of Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture (2014010), Qingdao Civil Science and Technology Project (14-2-3-34-nsh).

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    1. Shandong Peanut Research Institute, Qingdao, 266100, People’s Republic of China

      Na Chen, Xiaoyuan Chi, Zhimeng Zhang, Lijuan Pan, Mingna Chen, Tong Wang, Mian Wang, Zhen Yang & Shanlin Yu

    2. Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao, 266001, People’s Republic of China

      Maowen Su

    3. Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, 430062, People’s Republic of China

      Xiaoyuan Chi

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    1. Na Chen
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    Chen, N., Su, M., Chi, X. et al. Transcriptome analysis reveals salt-stress-regulated biological processes and key pathways in roots of peanut (Arachis hypogaea L.). Genes Genom 38, 493–507 (2016). https://doi.org/10.1007/s13258-016-0395-5

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