Abstract
In this study, the whole transcriptome of the Pacific oyster Crassostrea gigas was sequenced using Illumina RNA-seq. De novo assembly was performed with 33,750,764 raw reads using Trinity, which assembled 87,887 contigs. Transdecoder found 41,542 candidate coding contigs which showed homology to other species by BLAST analysis. Functional gene annotation was performed by Gene Ontology and KEGG pathway analyses. Finally, we identified a number of expressed gene pathways for C. gigas representing a useful model animal for gene information-based study such as environmental monitoring, immune-relevant aquaculture, and ecotoxicogenomics studies to uncover molecular mechanisms of stress-triggered sensitivity and physiological response to C. gigas.
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Acknowledgments
This work was supported by a Grant (RP-2015-AG-089) funded to Hyun-Jeong Lim, National Institute of Fisheries Science of the Korean government.
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All the experiments were approved by the animal care and use committee of National Fisheries Research and Development Institute (NFRDI, Pusan, South Korea).
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All authors claim no conflicts of interest.
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Lim, HJ., Lim, JS., Lee, JS. et al. Transcriptome profiling of the Pacific oyster Crassostrea gigas by Illumina RNA-seq. Genes Genom 38, 359–365 (2016). https://doi.org/10.1007/s13258-015-0376-0
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DOI: https://doi.org/10.1007/s13258-015-0376-0