Abstract
Lagerstroemia indica is a popular woody ornamental plant throughout the world. However, relatively little is known about the molecular processes regulating leaf color in L. indica compared with other ornamental plants. Although yellow leaf mutants from various organisms have been well characterized, L. indica yellow leaf mutant has not yet been reported. In this study, a L. indica yellow leaf mutant, named YL03, was characterized and its leaf transcriptome was sequenced. A total of 30,712,752 reads were generated and assembled de novo into 45,308 unigenes with an average length of 987.51 bp. Among these unigenes, 21,339 (47.10 %) were identified as putative homologs of annotated sequences in public databases. A total of 79 unigenes involved in chlorophyll biosynthesis and degradation, photosynthesis and chloroplast development were identified. The expression levels of those genes were detected using quantitative real-time PCR in this study. Among those genes, 11 unigenes showed highly significant difference in the mutant compared to wild type plants. Conclusively, the leaf color formation is greatly affected by the activity of chloroplast development and chlorophyll metabolism. And the possible formation pathway of yellow leaf mutant is deduced based on our results.
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Acknowledgments
This work was supported by Transformational fund of agricultural scientific and technological achievements, Ministry of Science and Technology (2013GB2C100185), Public service platform of science and technology of Jiangsu province (BM2012058), Natural science funds of Jiangsu province (BK2012377), Agricultural independent innovation funds of Jiangsu province (CX[14]2032) and Modern agriculture project of Nanjing (201201021).
Authors’ contributions
The study was conceived by Peng Wang. The plant material preparation was carried out by Shu’an Wang, Lingling Ma and Linfang Li. Data analysis and bioinformatics analysis was finished by Rutong Yang, Yuzhu Ma and Qing Wang. Manuscript was written by Ya Li and Zhenyu Zhang. All authors had read and approved the final manuscript.
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Ya Li and Zhenyu Zhang have contributed equally to this work.
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Supplementary material 2 (XLS 31 kb)Table S2: The candidate uingenes involved in chlorophyll biosynthesis and degradation
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Supplementary material 6 (XLS 28 kb)Table S6: The summary of 11 unigenes showing significant difference between mutant type and wild type by qRT-PCR
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Supplementary material 7 (DOC 33 kb)Table S7: The qRT-PCR analysis of 11 candidate uingenes in the mutant from May 3 to August 2
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Supplementary material 8 (TIFF 367 kb)Figure S1 - COG functional classifications of mutant type. In total, 11,512 (25.41 %) unigenes were assigned to 25 COG classifications. A: signal transduction mechanisms; B: general function prediction only; C: posttranslational modification, protein turnover, chaperones; D: translation, ribosomal structure and biogenesis; E: Intracellular trafficking, secretion, and vesicular transport; F: Carbohydrate transport and metabolism; G: function unknown; H: energy production and conversion; I: transcription; J: secondary metabolites biosynthesis, transport and catabolism; K: amino acid transport and metabolism; L: lipid transport and metabolism; M: RNA processing and modification; N: cytoskeleton; O: inorganic ion transport and metabolism; P: replication, recombination and repair; Q: cell cycle control, cell division, chromosome partitioning; R: cell wall/membrane/envelope biogenesis; S: coenzyme transport and metabolism; T: nucleotide transport and metabolism; U: chromatin structure and dynamics; V: defense mechanisms; W:nuclear structure; X: extracellular structures; Y: cell motility
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Li, Y., Zhang, Z., Wang, P. et al. Comprehensive transcriptome analysis discovers novel candidate genes related to leaf color in a Lagerstroemia indica yellow leaf mutant. Genes Genom 37, 851–863 (2015). https://doi.org/10.1007/s13258-015-0317-y
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DOI: https://doi.org/10.1007/s13258-015-0317-y