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Proteome alterations of reverse photoperiod-sensitive genic male sterile rice (Oryza sativa L.) at fertility transformation stage

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Abstract

The two-line system has been widely used in hybrid rice seed production, and the reverse photoperiod-sensitive genic male sterile line is a new germplasm with an opposite phenotype compared with normal photoperiod-sensitive genic male sterile rice. To better understand the molecular mechanisms of fertility regulation in reverse photoperiod-sensitive genic male sterile rice, a comparative proteomic approach was used to analyze the protein profiles of three different tissues (young panicles, flag leaves and leaf sheaths) of D52S during the sensitive period of pollen fertility transformation under sterile and fertile conditions. By quantitative analysis, 66 protein spots were identified to be significantly changed in the three tissues. Bioinformatics analyses revealed that in sterile rice, a number of proteins involved in lignin-flavonoid biosynthesis pathway were down-accumulated in panicles. The majority of proteins associated with energy metabolism were down-accumulated in leaf sheaths while the proteins up-accumulated in leaves and leaf sheaths were exclusively photosynthesis and defense related. Based on the proteomics data, a short-day induced male sterility protein network was proposed. In addition, the genes of selected protein spots were further analyzed by qPCR. These findings provide data for better understanding the regulation of pollen fertility in reverse photoperiod-sensitive genic male sterile rice, which could assist in the development of practical reverse photoperiod-sensitive genic male sterile rice for large-scale crop breeding programs.

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Abbreviations

β-met:

Beta-mercaptoethanol

LD:

Long-day length

SD:

Short-day length

RPGMS:

Reverse photoperiod-sensitive genic male sterile

EGMS:

Environmentally sensitive genetic male sterile

PGMS:

Photoperiod-sensitive genic male sterile

TGMS:

Thermo-sensitive genic male sterile

NPK:

N:P2O5:K2O

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 31371600).

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The authors have declared no conflict of interest.

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Authors and Affiliations

  1. MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Zhen Chen, Haixia Li, Xueli Ma, Hongxia Dong, Dong Ma & Hanlai Zeng

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  1. Zhen Chen
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  2. Haixia Li
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  3. Xueli Ma
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  4. Hongxia Dong
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  5. Dong Ma
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Correspondence to Hanlai Zeng.

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Supplementary Fig. S1

The experimental design of this study. (TIFF 4,960 kb)

Supplementary Fig. S2

Close-up views of the regions showing the distribution of some differentially accumulated protein spots. In each panel, protein patterns of D52S under SD (left) and LD (middle) conditions are shown. Differentially accumulated protein spots are indicated by black arrows. The quantitative analysis of each protein spot is presented in the right column. The data are the mean values ±SEM (n = 3). (TIFF 3,403 kb)

Supplementary Fig. S3

The category distribution of the identified protein spots from young panicles, leaf sheaths and leaves analysed by WEGO. (TIFF 913 kb)

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Supplementary material 5 (PDF 3,403 kb)

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Chen, Z., Li, H., Ma, X. et al. Proteome alterations of reverse photoperiod-sensitive genic male sterile rice (Oryza sativa L.) at fertility transformation stage. Genes Genom 36, 711–726 (2014). https://doi.org/10.1007/s13258-014-0205-x

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