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Identification, fine mapping and characterization of Rht-dp, a recessive wheat dwarfing (reduced height) gene derived from Triticum polonicum

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Abstract

Semi-dwarfism is an agronomically important trait in breeding for resistance to damage by wind and rain (lodging resistance) and for stable high yields. Dwarf Polish wheat (Triticum polonicum L., 2n = 4x = 28, AABB AS304) is a potential donor of dwarfing and other traits for common wheat improvement. A genetic analysis using an F2 population derived from a cross of AS304 and tall cultivar AS302 and derived F2:3 lines indicated that AS304 carries a recessive dwarfing gene, temporarily designated Rht-dp. Molecular markers and bulked segregant analysis were used to characterize and map the gene. Eight polymorphic SSR markers (Xwmc511, Xgwm495, Xgwm 113, Xgwm192, Xgpw7026, Xgpw3017, Xgpw1108 and Xgpw7521) on chromosome arm 4BS and two AFLP markers (M 8 /E 5 and M 4 /E 3 ) were mapped relative to the dwarfing locus. The closest linked markers, Xgpw3017 and M 8 /E 5 at 0.5 and 3.5 cM, respectively, from Rht-dp will enable its marker assisted transfer to wheat breeding populations. Allelic tests indicated that Rht-dp was allelic to Rht-B1b; hence it may be an alternative allele at the Rht-B1 locus.

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Authors and Affiliations

  1. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, Sichuan, China

    Hou-Yang Kang, Li-Juan Lin, Zhi-Jian Song, Jing-Ya Yuan, Mei-Yu Zhong, Hai-Qin Zhang, Xing Fan, Li-Na Sha, Yi Wang, Li-Li Xu & Yong-Hong Zhou

  2. Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Yaan, 625014, Sichuan, China

    Hou-Yang Kang & Yong-Hong Zhou

  3. College of Resources and Environmemt, Sichuan Agricultural University, Wenjiang, 611130, Chengdu, Sichuan, China

    Jian Zeng

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  1. Hou-Yang Kang
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  2. Li-Juan Lin
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Correspondence to Yong-Hong Zhou.

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H.-Y. Kang and L.-J. Lin contributed equally to this work.

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Kang, HY., Lin, LJ., Song, ZJ. et al. Identification, fine mapping and characterization of Rht-dp, a recessive wheat dwarfing (reduced height) gene derived from Triticum polonicum . Genes Genom 34, 509–515 (2012). https://doi.org/10.1007/s13258-012-0022-z

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