Protein & Cell

, Volume 1, Issue 5, pp 453–458

Crystal structure of a novel non-Pfam protein PF2046 solved using low resolution B-factor sharpening and multi-crystal averaging methods

Authors

  • Jing Su
    • National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of Sciences
  • Yang Li
    • National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of Sciences
  • Neil Shaw
    • National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of Sciences
  • Weihong Zhou
    • College of Life SciencesNankai University
  • Min Zhang
    • School of Life SciencesAnhui University
  • Hao Xu
    • Department of Biochemistry and Molecular BiologyUniversity of Georgia
  • Bi-Cheng Wang
    • Department of Biochemistry and Molecular BiologyUniversity of Georgia
    • National Laboratory of Biomacromolecules, Institute of BiophysicsChinese Academy of Sciences
Communication

DOI: 10.1007/s13238-010-0045-7

Cite this article as:
Su, J., Li, Y., Shaw, N. et al. Protein Cell (2010) 1: 453. doi:10.1007/s13238-010-0045-7

Abstract

Sometimes crystals cannot diffract X-rays beyond 3.0 Å resolution due to the intrinsic flexibility associated with the protein. Low resolution diffraction data not only pose a challenge to structure determination, but also hamper interpretation of mechanistic details. Crystals of a 25.6 kDa non-Pfam, hypothetical protein, PF2046, diffracted X-rays to 3.38 Å resolution. A combination of Se-Met derived heavy atom positions with multiple cycles of B-factor sharpening, multi-crystal averaging, restrained refinement followed by manual inspection of electron density and model building resulted in a final model with a R value of 23.5 (Rfree= 24.7). The asymmetric unit was large and consisted of six molecules arranged as a homodimer of trimers. Analysis of the structure revealed the presence of a RNA binding domain suggesting a role for PF2046 in the processing of nucleic acids.

Keywords

low resolution diffractionPF2046Bfactor sharpeninga homodimer of trimers

Copyright information

© Higher Education Press and Springer-Verlag Berlin Heidelberg 2010