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Diverse species of Colletotrichum associated with grapevine anthracnose in China

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Abstract

Grapevine anthracnose is an important disease, responsible for mild to severe yield losses in grape production, and is also an important post harvest disease. The disease was studied in vineyards in six provinces in China, with 34 isolates obtained from diseased grapes. Multi-gene (ACT, ITS, GAPDH, TUB2 and CHS) analysis coupled with morphology showed that Colletotrichum aenigma, C. hebeiense sp. nov. and C. viniferum were associated with grapevine anthracnose in China. Colletotrichum aenigma is reported for the first time as associated with grapevine anthracnose. Colletotrichum hebeiense is a new species introduced here. Pathogenicity testing showed that all species can infect grapes, causing anthracnose however, virulence of species and isolates showed great variation. Phylogenetic analysis showed that C. viniferum is a cryptic species and its taxonomy needs to be resolved in the future.

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Acknowledgments

The study is funded by CARS-30. There are many people to thank for their assistance with this work. The authors would like to thank Miss. Qin Tian for the help given to perform the pathogenicity tests and Miss. K. W. T. Chethana for the help given in phylogenetic analysis. Authors would like to thank the grape cultivators who provided the diseased fruits. K.D. Hyde thanks the National Research Council of Thailand, Colletotrichum grant number 54201020003 and The Chinese Academy of Sciences, project number 2013T2S0030, for the award of Visiting Professorship for Senior International Scientists at Kunming Institute of Botany. This work was also supported by a grant from the National Plan of Science and Technology, King Abdulaziz City of Science and Technology, Riyadh, Saudi Arabia (10-Bio-965-02). M.M.R.S. Jayawardena would like to thank H. A. Ariyawansa, D. Udayanga for the support given.

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Authors and Affiliations

  1. Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, People’s Republic of China

    Ji-Ye Yan, M. M. R. S. Jayawardena, Wei Zhang, Mei Liu, Jin-Bao Huang, Jing-Jing Shang & Xing-Hong Li

  2. Institute of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    M. M. R. S. Jayawardena, Ishani D. Goonasekara & Kevin D. Hyde

  3. Department of Plant Pathology, Agriculture College, Guizhou University, Guiyang, 550025, People’s Republic of China

    Yong Wang

  4. Institute of Plant Protection, Chinese Academy of Agriculture Sciences, Beijing, 1000193, People’s Republic of China

    Zhong-Yue Wang

  5. Department of Plant Pathology, China Agricultural University, Beijing, 100193, People’s Republic of China

    You-Liang Peng

  6. College of Science, Botany and Microbiology Department, King Saud University, Riyadh, Saudi Arabia

    Ali Bahkali

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  1. Ji-Ye Yan
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Yan, JY., Jayawardena, M.M.R.S., Goonasekara, I.D. et al. Diverse species of Colletotrichum associated with grapevine anthracnose in China. Fungal Diversity 71, 233–246 (2015). https://doi.org/10.1007/s13225-014-0310-9

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