Skip to main content
SpringerLink
Log in

Species of Lasiodiplodia associated with papaya stem-end rot in Brazil

  • Published:
Fungal Diversity Aims and scope Submit manuscript

Abstract

This study aims to identify and characterize species of Lasiodiplodia associated with stem-end rot of papaya in six different populations in the Northeast of Brazil. Fungal identifications were made using a combination of morphology together with a phylogenetic analysis based on partial translation elongation factor 1-α sequence (EF-1α) and internal transcribed spacers (ITS). Five species of Lasiodiplodia were identified: Lasiodiplodia brasiliense sp. nov., L. hormozganensis, L. marypalme sp. nov., L. pseudotheobromae and L. theobromae. Only L. theobromae had previously been reported in papaya, while all the other species are reported for the first time in association with this host in Brazil and worldwide. Lasiodiplodia theobromae was the most prevalent species. All species of Lasiodiplodia were pathogenic on papaya fruit, with L. hormozganensis being the most virulent.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Abdollahzadeh J, Javadi A, Mohammadi EG, Zare R, Phillips AJL (2010) Phylogeny and morphology of four new species of Lasiodiplodia from Iran. Persoonia 25:1–10

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Al-Sadi AM, Al-Wehaibi AN, Al-Shariqi RM, Al-Hammadi MS, Al-Hosni IA, Al-Mahmooli IH, Al-Ghaithi AG (2013) Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, Citrus, and mango in Oman and the UAE. Plant Dis, Accepted for publication. doi:10.1094/PDIS-03–13–0245-RE

  • Alves A, Crous PW, Correia A, Phillips AJL (2008) Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Divers 28:1–13

    Google Scholar 

  • Begoude BAD, Slippers B, Wingfield MJ, Roux J (2010) Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar. Mycol Prog 9:101–123

    Article  Google Scholar 

  • Begoude BAD, Slippers B, Wingfield MJ, Roux J (2011) The pathogenic potential of endophytic Botryosphaeriaceous fungi on Terminalia species in Cameroon. Forest Pathol 41:281–292

    Article  Google Scholar 

  • Burgess TI, Barber PA, Mohali S, Pegg G, de Beer W, Wingfield MJ (2006) Three new Lasiodiplodia spp. from the tropics, recognized based on DNA sequence comparisons and morphology. Mycologia 98:423–435

    Article  PubMed  CAS  Google Scholar 

  • Correia KC, Câmara MPS, Barbosa MAG, Sales R Jr, Agustí-Brisach C, Gramaje D, García-Jiménez J, Abad-Campos P, Armengol J, Michereff SJ (2013) Fungal trunk pathogens associated with table grape decline in Northeastern Brazil. Phytopathol Mediterr 52:380–387

    Google Scholar 

  • Costa VSO, Michereff SJ, Martins RB, Gava CAT, Mizubuti ESG, Camara MPS (2010) Species of Botryosphaeriaceae associated on mango in Brazil. Eur J Plant Pathol 127:509–519

    Article  Google Scholar 

  • Damm U, Crous PW, Fourie PH (2007) Botryosphaeriaceae as potential pathogens of Prunus in South Africa, with descriptions of Diplodia africana and Lasiodiplodia plurivora sp. nov. Mycologia 99:664–680

    Article  PubMed  CAS  Google Scholar 

  • Dantas SAF, Oliveira SMA (2006) Doenças do mamão. In: Oliveira SMA, Terão D, Dantas SAF, Tavares SCCH (eds) Patologia pós-colheita: frutas, olerícolas e ornamentais tropicais. Embrapa Informação Tecnológica, Brasília, pp 695–729

    Google Scholar 

  • Dantas SAF, Oliveira SMA, Michereff SJ, Nascimento LC, Gurgel LMS, Pessoa WRLS (2003) Doenças fúngicas pós-colheita em mamões e laranjas comercializados na Central de Abastecimento do Recife. Fitopatol Bras 28:528–533

    Article  Google Scholar 

  • FAO (2013) FAOSTAT. http://faostat3.fao.org/home/index.html. Accessed 20 May 2013

  • Freire FCO, Cardoso JE, Viana FMP (2003) Doenças de fruteiras tropicais de interesse agroindustrial. Embrapa Informação Tecnológica, Brasília, 687 p

    Google Scholar 

  • Hillis DM, Bull JJ (1993) An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis. Syst Biol 42:182–192

    Article  Google Scholar 

  • Ismail AM, Cirvilleri G, Polizzi G, Crous PW, Groenewald JZ, Lombard L (2012) Lasiodiplodia species associated with dieback disease of mango (Mangifera indica) in Egypt. Australas Plant Pathol 41:649–660

    Article  Google Scholar 

  • Kumar DSS, Hyde KD (2004) Biodiversity and tissue-recurrence of endophytic fungi in Tripterygium wilfordii. Fungal Divers 17:69–90

    CAS  Google Scholar 

  • Liu JK, Phookamsak R, Doilom M, Wikee S, Li YM, Ariyawansha H, Boonmee S, Putarak Chomnunti P, Dai DQ, Bhat JD, Romero AI, Zhuang WY, Monkai J, Jones EBG, Chukeatirote E, Thida Win Ko Ko TW, Zhao YC, Wang Y, Hyde KD (2012) Towards a natural classification of Botryosphaeriales. Fungal Divers 57:149–210

    Article  Google Scholar 

  • Marques MW, Lima NB, Morais Junior MA, Barbosa MAG, Souza BO, Michereff SJ, Phillips AJL, Câmara MPS (2013) Species of Lasiodiplodia associated with mango in Brazil. Fungal Divers 61:181–193

    Article  Google Scholar 

  • Ministério da Integração Nacional (2000) Frutiséries: mamão. site. http://www.bnb.gov.br/content/Aplicacao/ETENE/Rede_Irrigacao/Docs/FrutiSeries-MG_7_Mamao.PDF. Accessed 15 February 2013

  • Page RDM (1996) TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12:357–358

    PubMed  CAS  Google Scholar 

  • Paull RE, Nishijima W, Reyes M, Cavaletto CC (1997) Postharvest handling and losses during marketing of papaya (Carica papaya L.). Postharvest Biol Tec 11:165–179

    Article  Google Scholar 

  • Pavlic D, Slippers B, Coutinho TA, GryzenhoutM WMJ (2004) Lasiodiplodia gonubiensis sp. nov., a new Botryosphaeria anamorph from native Syzygium cordatum in South Africa. Stud Mycol 50:313–322

    Google Scholar 

  • Pavlic D, Wingfield MJ, Barber P, Slippers B, Harder GES, Burgess TI (2008) Seven new species of the Botryosphaeriaceae from baobab and other native trees in Western Australia. Mycologia 100:851–866

    Article  PubMed  Google Scholar 

  • Pereira AVS, Martins RB, Michereff SJ, Silva MB, Câmara MPS (2012) Sensitivity of Lasiodiplodia theobromae from Brazilian papaya orchards to MBC and DMI fungicides. Eur J Plant Pathol 132:489–498

    Article  CAS  Google Scholar 

  • Pérez CA, Wingfield MJ, Slippers B, Altier NA, Blanchette RA (2010) Endophytic and canker-associated Botryosphaeriaceae occurring on non-native Eucalyptus and native Myrtaceae trees in Uruguay. Fungal Divers 41:53–69

    Article  Google Scholar 

  • Phillips AJL (2006) The Botryosphaeria site. http://www.crem.fct.unl.pt/botryosphaeria_site. Accessed 08 April 2012

  • Phillips AJL, Alves A, Correia A, Luque J (2005) Two new species of Botryosphaeria with brown, 1-septate ascospores and Dothiorella anamorphs. Mycologia 97:513–529

    Article  PubMed  Google Scholar 

  • Phillips AJL, Alves A, Pennycook SR, Johnston PR, Ramaley A, Akulov A, Crous PW (2008) Resolving the phylogenetic and taxonomic status of dark-spored teleomorph genera in the Botryosphaeriaceae. Persoonia 21:29–55

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Phillips AJL, Alves A, Abdollahzadeh J, Slippers B, Wingfield MJ, Groenewald JZ, Crous PW (2013) The Botryosphaeriaceae: genera and species known from culture. Stud Mycol 76:51–167

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Punithalingam E (1976) Botryodiplodia theobromae. CMI Descriptions of pathogenic fungi and bacteria, No. 519. Commonwealth Mycological Institute, Kew, 2 p

    Google Scholar 

  • Punithalingam E (1980) Plant diseases attributed to Botryodiplodia theobromae Pat. Cramer, Vaduz, 123 p

    Google Scholar 

  • Rannala B, Yang Z (1996) Probability distribution of molecular evolutionary trees: a new method of phylogenetic inference. J Mol Evol 43:304–311

    Article  PubMed  CAS  Google Scholar 

  • Rayner RW (1970) A mycological colour chart. Commonwealth Mycological Institute and British Mycological Society, Kew, 34 p

    Google Scholar 

  • Ronquist F, Huelsenbeck JP (2003) MrBayes3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19:1572–1574

    Article  PubMed  CAS  Google Scholar 

  • Sakalidis ML, Ray JD, Lanoiselet V, Hardy GESJ, Burgess TI (2011) Pathogenic Botryosphaeriaceae associated with Mangifera indica in the Kimberley Region of Western Australia. Eur J Plant Pathol 130:379–391

    Article  Google Scholar 

  • Shannon CE, Weaver W (1949) The mathematical theory of communication. University of Illinois Press, Urbana, 144 p

    Google Scholar 

  • Slippers B, Wingfield MJ (2007) Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biol Rev 21:90–106

    Article  Google Scholar 

  • Slippers B, Crous PW, Denman S, Coutinho TA, Wingfield BD, Wingfield MJ (2004) Combined multiple gene genealogies and phenotypic characters differentiate several species previously identified as Botryosphaeria dothidea. Mycologia 96:83–101

    Article  PubMed  CAS  Google Scholar 

  • Slippers B, Boissin E, Phillips AJL, Groenewald JZ, Lombard L, Wingfield MJ, Postma A, Burgess T, Crous PW (2013) Phylogenetic lineages in the Botryosphaeriales: a systematic and evolutionary framework. Stud Mycol 76:31–49

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Sutton BC (1980) The Coelomycetes: fungi imperfecti with pycnidia, acervuli and stromata. Commonwealth Mycological Institute, Kew, 696 p

    Google Scholar 

  • Swofford DL (2003) PAUP: Phylogenetic analysis using parsimony (*and other methods) Version 4. Sinauer Associates, Sunderland

    Google Scholar 

  • Tavares SCCH (2002) Epidemiologia e manejo integrado de Botryodiplodia theobromae - situação atual no Brasil e no mundo. Fitopatol Bras 27:46–52

    Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Urbéz-Torres JR, Peduto F, Striegle RK, Urrea-Romer JEO, Rupe JC, Cartwright RD, Gubler WD (2012) Characterization of fungal pathogens associated with grapevine trunk diseases in Arkansas and Missouri. Fungal Divers 52:169–189

    Article  Google Scholar 

  • Ventura JA, Costa H, Tatagiba JS (2004) Papaya diseases and integrated control. In: Naqvi SAMH (ed) Diseases of fruits and vegetables, vol 2. Kluwer, Dordrecht, pp 201–268

    Google Scholar 

  • von Arx JA (1987) Plant pathogenic fungi. J. Cramer, Berlin, 288 p

    Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols, a guide to methods and applications. Academic, San Diego, pp 315–322

    Chapter  Google Scholar 

  • Wikee S, Lombard L, Nakashima C, Motohashi K, Chukeatirote E, Cheewangkoon R, McKenzie EHC, Hyde KD, Crous PW (2013) A phylogenetic re-evaluation of Phyllosticta (Botryosphaeriales). Stud Mycol 76:1–29

    Article  PubMed  CAS  PubMed Central  Google Scholar 

  • Wright AF, Harmon PF (2010) Identification of species in the Botryosphaeriaceae family causing stem blight on southern highbush blueberry in Florida. Plant Dis 94:966–971

    Article  Google Scholar 

  • Young ND, Healey J (2003) GapCoder automates the use of indel characters in phylogenetic analysis. BMC Bioinformatics 4:6, http://www.biomedcentral.com/content/pdf/1471–2105–4–6.pdf

  • Zak JC, Willig MR (2004) Fungal biodiversity patterns. In: Mueller GM, Bills GF, Foster MS (eds) Biodiversity of fungi: inventory and monitoring methods. Elsevier Academic, Amsterdan, pp 59–75

    Chapter  Google Scholar 

  • Zhao JP, Lu Q, Liang J, Decock C, Zhang XY (2010) Lasiodiplodia pseudotheobromae, a new record of pathogenic fungus from some subtropical and tropical trees in southern China. Cryptogamie Mycol 31:431–439

    Google Scholar 

Download references

Acknowledgments

This work was financed by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 149920/2012-1). M. P. S. Câmara, and S. J. Michereff also acknowledge the CNPq research fellowship. A.J.L. Phillips thanks Fundação para a Ciência e a Tecnologia (Portugal) for financial support through grant PEst-OE/BIA/UI0457/2011

Author information

Authors and Affiliations

  1. Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900, Recife, Pernambuco, Brazil

    Mariote S. B. Netto, Marília W. Marques, Waléria G. Lima, Sami J. Michereff & Marcos P. S. Câmara

  2. Centro de Ciências Agrárias, Universidade Federal de Alagoas, 57100-000, Rio Largo, Alagoas, Brazil

    Iraíldes P. Assunção, Gaus S. A. Lima & Jean H. A. Monteiro

  3. Departamento de Genética, Universidade Federal de Pernambuco, 50732-970, Recife, Brazil

    Valdir de Queiroz Balbino

  4. Centro de Recursos Microbiológicos, Departamento de Ciências da Vida, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516, Caparica, Portugal

    Alan J. L. Phillips

Authors
  1. Mariote S. B. Netto
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Iraíldes P. Assunção
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gaus S. A. Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Marília W. Marques
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Waléria G. Lima
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jean H. A. Monteiro
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Valdir de Queiroz Balbino
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sami J. Michereff
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Alan J. L. Phillips
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Marcos P. S. Câmara
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Marcos P. S. Câmara.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Netto, M.S.B., Assunção, I.P., Lima, G.S.A. et al. Species of Lasiodiplodia associated with papaya stem-end rot in Brazil. Fungal Diversity 67, 127–141 (2014). https://doi.org/10.1007/s13225-014-0279-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13225-014-0279-4

Keywords

Search

Navigation