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Fluorescence Spectroscopy: A New Approach in Cervical Cancer

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Abstract

Objective

To study the diagnostic potential of fluorescence spectroscopy and its comparison with different screening methods, including Pap smear and colposcopy, in detecting early cervical neoplasia.

Method

The study was conducted on patients with gynecological complaints. A full gynecological workup of the patients was done along with Pap smear and colposcopy. Cervical biopsy was done in suspected cases and fresh tissue was sent to IIT for spectroscopy.

Result

There is a definite increase in NADH fluorescence (67.4 %) and a decrease in collagen fluorescence (74 %) in dysplastic tissues. When epithelial fluorescence and stromal fluorescence are considered together, diagnostic accuracy is increased to 96.5 %.

Conclusion

The clinical diagnosis of cervical neoplasia by spectroscopic methods is potentially a reliable, fast, and cost-effective alternative to the conventional smear test which needs trained personnel for its interpretation. Research is still continuing to obtain a statistically significant cutoff value from in vitro studies and then use them for in vivo study.

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Correspondence to Kiran Pandey.

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Pandey, K., Pradhan, A., Agarwal, A. et al. Fluorescence Spectroscopy: A New Approach in Cervical Cancer. J Obstet Gynecol India 62, 432–436 (2012). https://doi.org/10.1007/s13224-012-0298-6

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  • DOI: https://doi.org/10.1007/s13224-012-0298-6

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