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Determination of an antimicrobial activity of Weissella confusa, Lactobacillus fermentum, and Lactobacillus plantarum against clinical pathogenic strains of Escherichia coli and Staphylococcus aureus in co-culture

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Abstract

Lactic acid bacteria (LAB) have long been used to produce safe and high quality products as they are potential producers of a wide range of antimicrobial compounds that exert either narrow or wide spectrum antimicrobial activity towards spoilage or disease-causing organisms. The present investigation aimed to study the antimicrobial effect of three LAB strains, viz., Lactobacillus plantarum (86), Lactobacillus fermentum (AI2) and Weissella confusa (AI10), against two clinical pathogenic strains viz., Escherichia coli NG 502121 and Staphylococcus aureus AY 507047 in co-culture. Effects of change in inoculum size, and growth measurement at different time intervals were also studied. The pH and viable count were measured for initial as well as 24 h incubated samples. A significant (P < 0.05) reduction (2–3 log cycles) in growth of both pathogens while co-cultured with LAB strains was observed. The nonsignificant (P < 0.05) pH difference revealed the action of other metabolites apart from organic acids. LAB strains overruled the growth of E. coli and S. aureus within 10 and 6 h of the initial growth stage, respectively, compared to controls. These results led us to further characterize and purify the antimicrobial compound produced by the studied strains, so that they can be exploited in the production of safe foods with longer shelf life.

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References

  • Anas M, Eddine HJ, Mebrouk K (2008) Antimicrobial activity of lactobacillus species isolated from Algerian raw goat’s milk against S. aureus. W J Dairy Food Sci 3(2):39–49

    Google Scholar 

  • Aoki SK, Pamma R, Hernday AD, Bickham JE, Braaten BA, Low DA (2005) Contact dependent inhibition of growth in Escherichia coli. Science 309:1245–1248

    Article  CAS  PubMed  Google Scholar 

  • Ballal M, Shivananda PM (2002) Rotavirus and enteric pathogens in infantile diarrhoea in Manipal, South India. Indian J Pediatr 69:393–396

    Article  PubMed  Google Scholar 

  • Celine Delbes-Paus C, Dorchies G, Chaabna Z, Callon C, Montel MC (2010) Contribution of hydrogen peroxide to the inhibition of S. aureus by Lc. garvieae in interaction with raw milk microbial community. Food Microbiol 27:924–932

    Article  PubMed  Google Scholar 

  • Charlier C, Cretenet M, Even S, Le Loir Y (2009) Interactions between Staphylococcus aureus and lactic acid bacteria: an old story with new perspectives. Int J Food Microbiol 131:30–39

    Article  CAS  PubMed  Google Scholar 

  • Davoodabadi A, Dallal MMS, Foroushani AR, Douraghi M, Yazdi MKS, Harati FA (2015) Antibacterial activity of Lactobacillus spp. isolated from the feces of healthy infants against enteropathogenic bacteria. Anaerobe 34:53–58

    Article  PubMed  Google Scholar 

  • Deng K, Chen T, Wu Q, Xin H, Wei Q, Hu P, Wang X, Wang X, Wei H, Shah NP (2015) In vitro and in vivo examination of anticolonization of pathogens by Lactobacillus paracasei FJ861111.1. J Dairy Sci 98(10):6759–66

    Article  CAS  PubMed  Google Scholar 

  • Drago L, Gismondo MR, Lombardi A, De Haen C, Gozzini L (1997) Inhibition of in vitro growth of enteropathogens by new Lactobacillus isolates of human intestinal origin. FEMS Microbiol Lett 153:455–63

    Article  CAS  PubMed  Google Scholar 

  • Fooks LJ, Gibson GR (2002) In vitro investigation of the effect of probiotics and prebiotics on selected human intestinal pathogens. FEMS Microbiol Ecol 39:67–75

    Article  CAS  PubMed  Google Scholar 

  • Gong HS, Meng XC, Wang H (2010) Plantaricin MG active against Gram-negative bacteria producedby Lactobacillus plantarum KLDS1.0391 isolated from ‘‘Jiaoke”, a traditional fermented cream from China.Food Control 21:89–96

  • Huang R, Tao X, Wan C, Li S, Xu H, Xu F, Shah NP, Wei H (2015) In vitro probiotic characteristics of Lactobacillus plantarum ZDY 2013 and its modulatory effect on gut microbiota of mice. J Dairy Sci 98(10):5850–61

    Article  CAS  PubMed  Google Scholar 

  • Karska-Wysocki B, Bazo M, Smoragiewicz W (2010) Antibacterial activity of Lactobacillus acidophilus and Lactobacillus casei against methicillin-resistant Staphylococcus aureus (MRSA). Microbiol Res 165:674–686

    Article  PubMed  Google Scholar 

  • Kumar A, Kumar D (2015) Characterization of Lactobacillus isolated from dairy samples for probiotic properties. Anaerol 33:117–23

    Article  CAS  Google Scholar 

  • Laughton MJ, Devillard E, Heinrich DE, Reid G, McCormick JK (2006) Inhibition of expression of a Staphylococcal superantigen-like protein by a soluble factor from L. reuteri. Microbiology 152:1155–1167

    Article  CAS  PubMed  Google Scholar 

  • Le loir Y, Baron F, Gautier M (2003) Staphylococcus aureus and food poisoning. Genet Mol Res 2:63–76

    PubMed  Google Scholar 

  • Liu H, Gao Y, Yu LR, Jones RC (2011) Inhibition of Staphylococcus aureus by lysostaphin expressing Lactobacillus plantarum WCFS1 in a modified genital tract secretion medium. Appl Environ Microbiol 77(24):8500–8508

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Mohammedsaeed W, McBain AJ, Cruickshank SM, O’Neill CA (2014) Lactobacillus rhamnosus GG inhibits the toxic effects of S. aureus on Epidermal Keratinocytes. Appl Environ Microbiol 80(18):5773–81

    Article  PubMed  PubMed Central  Google Scholar 

  • Nawaz SK, Riaz S, Hasnain S (2009) Screening for antimethicillin resistant Staphylococcus aureus (MRSA) bacteriocin producing bacteria. Afr J Biochem Res 8(3):365–368

    Google Scholar 

  • Ogunshe AAO, Omotoso MA, Adeyeye JA (2007) In vitro antimicrobial characteristics of bacteriocin producing Lactobacillus strains from Nigerian indigenous fermented foods. Afr J Biochem Res 6(4):445–453

    Google Scholar 

  • Pal A, Ramana KV (2010) Purification and characterization of bacteriocin from Weissella paramesenteroides dfr-8, an isolate from cucumber (cucumis sativus). J Food Biochem 34:932–948

    Article  CAS  Google Scholar 

  • Patel A, Lindstrom C, Patel A, Holst O, Prajapati JB (2012) Probiotic properties of exopolysaccharide producing lactic acid bacteria isolated from vegetables and traditional Indian fermented foods. Int J Ferm Foods 1(1):87–101

    Google Scholar 

  • Patel A, Falck P, Shah N, Immerzeel P, Adlercreutz P, Stalbrand H, Prajapati JB, Holst O, Nordberg Karlsson E (2013a) Evidence for xylooligosaccharide utilization in Weissella strains isolated from Indian fermented foods and vegetables. FEMS Microbiol Lett 346:20–28

    Article  CAS  PubMed  Google Scholar 

  • Patel A, Shah N, Ambalam P, Prajapati JB, Holst O, Ljungh A (2013b) Antimicrobial profile of lactic acid bacteria isolated from vegetables and indigenous fermented foods of India against clinical pathogens using microdilution method. Biomed Environ Sci 26(9):759–764

    CAS  PubMed  Google Scholar 

  • Patra A, Sil J, Das BK (2011) Isolation and characterization of dominant lactic acid bacteria from dahi at Medinipur and evaluation of their antibacterial activity. Int J Food Safety 13:157–163

    Google Scholar 

  • Pinto MGV, Franz CMAP, Schillinger U, Holzapfel WH (2006) Lactobacillus spp. with in vitro probiotic properties from human faeces and traditional fermented products. Int J Food Microbiol 109(3):205–214

    Article  Google Scholar 

  • Poppi LB, Rivaldi JD, Coutinhyo TS, Astolfi-Ferreira CS, Piantino-Ferreira AJ, Mancilha IM (2015) Effect of Lactobacillus spp. isolates supernatant on E. coli O157:H7 enhances the role of organic acids production as a factor for pathogen control. Pesqui Vet Bras 35(4):353–59

    Article  Google Scholar 

  • Powell JE, Witthuhn RC, Todorov SD, Dicks LMT (2007) Characterization of bacteriocin st8kf produced by a kefir isolate Lactobacillus plantarum st8kf. Int Dairy J 17(3):190–198

    Article  CAS  Google Scholar 

  • Robin DD, Sabrina V, Isabelle D, Christopher M, Andre T, Philippe TJ (2011) In vitro antagonistic activity evaluation of lactic acid bacteria (LAB) combined with cellulase enzyme against Campylobacter jejuni growth in co-culture. Microb Biotechnol 21(1):62–70

    Article  Google Scholar 

  • Ruiz-Barba JL, Guerrero BC, Barragan AM, Diaz RJ (2010) Co-culture with specific bacteria enhances survival of Lactobacillus plantarum NC8, an auto inducer-regulated bacteriocin producer, in olive fermentations. Food Microbiol 27(3):413–417

    Article  CAS  PubMed  Google Scholar 

  • Savino F, Cordisco L, Tarasco V, LoCatelli E, Gioia DD, Oggero R, Matteuzzi D (2011) Antagonistic effect of Lactobacillus strains against gas-producing coliforms isolated from colicky infants. BMC Microbiol 11:157

    Article  PubMed  PubMed Central  Google Scholar 

  • Sreekumar O, Hosono A (2000) Immediate effect of Lactobacillus acidophilus on the intestinal flora and fecal enzymes of rats and the In Vitro Inhibition of Escherichia coli in Co-culture. J Dairy Sci 83:931–939

    Article  CAS  PubMed  Google Scholar 

  • Tambekar DH, Bhutada SA (2010) An evaluation of probiotic potential of lactobacillus sp. from milk of domestic animals and commercial available probiotic preparations in prevention of enteric bacterial infections. Recent Res Sci Technol 2(10):82–88

    Google Scholar 

  • Turner MS, Lo R, Giffard PM (2007) Inhibition of S. aureus growth on tellurite-containing media by L. reuteri is dependent on CyuC and Thiol production. Appl Environ Microbiol 73(3):1005–1009

    Article  CAS  PubMed  Google Scholar 

  • Varma P, Dinesh KR, Menon KK, Biswas R (2010) l. fermentum isolated from human colonic mucosal biopsy inhibits the growth and adhesion of enteric and foodborne pathogens. J Food Sci 75:546–551

    Article  Google Scholar 

  • Vermeiren L, Devlieghere F, Debevere J (2006) Co-culture experiments demonstrate the usefulness of Lactobacillus sakei 10A to prolong the shelf-life of a model cooked ham. Int J Food Microbiol 108:68–77

    Article  CAS  PubMed  Google Scholar 

  • Yun JH, Lee KB, Sung YK, Kim EB, Lee HG, Choi YJ (2009) Isolation and characterization of potential probiotic lactobacilli from pig feces. J Basic Microbiol 49:220–226

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Dairy Microbiology, SMC College of Dairy Science, Anand Agricultural University, Anand, Gujarat, India

    Nihir Shah, Ami Patel & Jashbhai Prajapati

  2. Department of Biotechnology, Lund University, Lund, Sweden

    Nihir Shah, Ami Patel & Olle Holst

  3. Department of Medical Microbiology, Lund University, Lund, Sweden

    Nihir Shah, Ami Patel, Padma Ambalam & Asa Ljungh

  4. Department of Dairy and Food Microbiology, Mansinhbhai Institute of Dairy and Food Technology-MIDFT, Dudhsagar Dairy campus, Mehsana, 384 002, Gujarat, India

    Nihir Shah

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  1. Nihir Shah
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  2. Ami Patel
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  3. Padma Ambalam
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  4. Olle Holst
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  5. Asa Ljungh
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  6. Jashbhai Prajapati
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Correspondence to Nihir Shah.

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Shah, N., Patel, A., Ambalam, P. et al. Determination of an antimicrobial activity of Weissella confusa, Lactobacillus fermentum, and Lactobacillus plantarum against clinical pathogenic strains of Escherichia coli and Staphylococcus aureus in co-culture. Ann Microbiol 66, 1137–1143 (2016). https://doi.org/10.1007/s13213-016-1201-y

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  • DOI: https://doi.org/10.1007/s13213-016-1201-y

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