Abstract
In order to reduce the pollution and nitrogen loss resulting from ammonia emission during the composting process, a genetically stable microbial community, CC-E, capable of ammonia removal was enriched from cow feces using restrictive culture. The microbial diversity of CC-E was also investigated by 16S rRNA gene clone technology. Moreover, the effect of CC-E on composting inoculation was investigated by detecting the amount of ammonia produced. The results showed that the amount of ammonia produced from the treatments with the microbial community CC-E was 63 % lower (151.11 mg/kg) relative to the control (447.87 mg/kg) over the course of the 20-day culture period. The bacterial groups represented in the CC-E clone library were the gammaproteobacteria (16.7 %), betaproteobacteria (54.4 %), alphaproteobacteria (21.1 %), and uncultured compost bacterium (7.8 %). Furthermore, Sphingomonas sp., Serratia sp. and Alcaligenes sp. were isolated from the microbial community CC-E with ammonia removal capacity. It was found that ammonia emission was reduced to 60.7 % following CC-E inoculation during the first 20 days of composting. Nitrogen conservation was also influenced by inoculation. Results indicate that microbial community CC-E can be a useful resource for biological deodorization.
Similar content being viewed by others
References
Burgess RM, Pelletier MC, Ho KT, Serbst JR, Ryba SA, Kuhn A, Perron MM, Raczelowski P, Cantwell MG (2003) Removal of ammonia toxicity in marine sediment TIEs: a comparison of Ulva lactuca, zeolite and aeration methods. Mar Pollut Bull 46:607–618
Chen XG, Geng AL, Yan R, Gould WD, Ng YL, Liang DT (2004) Isolation and characterization of sulphur-oxidizing Thiomonas sp. and its potential application in biological deodorization. Lett Appl Microbiol 39:495–503
Gong WX, Wang SG, Sun XF, Liu XW, Yue QY, Gao BY (2008) Bioflocculant production by culture of Serratia ficaria and its application in wastewater treatment. Bioresour Technol 99:4668–4674
Guštin S, Marinšek-Logar R (2011) Effect of pH, temperature and air flow rate on the continuous ammonia stripping of the anaerobic digestion effluent. Process Saf Environ 89:61–66
Ho KL, Chung YC, Tseng CP (2008) Continuous deodorization and bacterial community analysis of a biofilter treating nitrogen-containing gases from swine waste storage pits. Bioresour Technol 99:2757–2765
Hort C, Gracy S, Platel V, Moynault L (2009) Evaluation of sewage sludge and yard waste compost as a biofilter media for the removal of ammonia and volatile organic sulfur compounds (VOSCs). Chem Eng J 152:44–53
Jian BQ, Zhu SP, Deng CY, Wu L, Shi HF, Gao ZG, Shi TL (2006) Characteristics of ammonia and hydrogen sulfide volatilization during composting of pig manure and screening of deodorizing microorganisms. T Chin Soc Agric Eng 22:183–186
Kim NJ, Sugano Y, Hirai M, Shoda M (2000) Removal of a high load of ammonia gas by a marine bacterium, Vibrio alginolyticus. J Biosci Bioeng 90:410–415
Lee DH, Lau AK, Pinder KL (2001) Development and performance of an alternative biofilter system. J Air Waste Manag Assoc 51:78–85
Lin M, You CB (1987) Denitrification and nitrogen fixation by Alcaligenes faecalis. Acta Agr Nucl Sin 1:3–10
Ohta Y, Ikeda M (1978) Deodorization of pig feces by actinomycetes. Appl Environ Microbiol 36:487–491
Pagans E, Barrena R, Font X, Sanchez A (2006) Ammonia emissions from the composting of different organic wastes. Dependency on process temperature. Chemosphere 62:1534–1542
Pattey E, Trzcinski MK, Desjardins RL (2005) Quantifying the reduction of greenhouse gas emissions as a result of composting dairy and beef cattle manure. Nutr Cycl Agroecosys 72:173–187
Patureau D, Zumstein E, Delgenes JP, Moletta R (2000) Aerobic denitrifiers isolated from diverse natural and managed ecosystems. Microb Ecol 39:145–152
Wang WD, Wang XF, Piao Z, Liu CL, Gao XZ, Cui ZJ (2007) Microbial dynamics during the composting process. Huan Jing Ke Xue 28:2591–2597
Wang Y, Zhou JB, Yang XY (2010) Effects of different long-term fertilization on the fractions of organic nitrogen and nitrogen mineralization in soils. Sci Agric Sin 43:1173–1180
Wang WD, Yan L, Cui Z, Gao Y, Wang Y, Jing R (2011a) Characterization of a microbial consortium capable of degrading lignocellulose. Bioresour Technol 102:9321–9324
Wang WD, Song YB, Wang YJ, Gao YM, Jing RY, Cui ZJ (2011b) Biodiversity of mesophilic microbial community BYND-8 capability of lignocellulose degradation and its effect on biogas production. Chin J Enviro Sci 32:253–258
Xue N, Wang Q, Wu C, Zhang L, Xie W (2010) Enhanced removal of NH3 during composting by a biotrickling filter inoculated with nitrifying bacteria. Biochem Eng J 51:86–93
Ye FX, Zhu RF, Ye YF (2008) Preparation of complex microbial adsorbent for deodorization and its application to deodorization. Trans Chin Soc Agric Eng 24:254–257
Ye FX, Zhu RF, Li Y (2009) Deodorization of swine manure slurry using horseradish peroxidase and peroxides. J Hazard Mater 167:148–153
Zhang FD, Zhang SQ, Wang YJ, Dou FG, Liu XM, Zou SW (2004) Deodorization techniques on fleeting continuous zymosis of organic feces with high temperature. J Agro-Environ Sci 23:796–800
Zhu H, Qu F, Zhu LH (1993) Isolation of genomic DNAs from plants, fungi and bacteria using benzyl chloride. Nucleic Acids Res 21:5279–5280
Acknowledgments
This work was supported by the Key Projects in the National Science & Technology Pillar Program during the 12th Five–Year Plan Period (2012BAD12B05), NSFC (31270536), Science Fund for Distinguished Young Scholars of Heilongjiang Province (LBH–QO8O27), Key Science and Technology Program of Heilongjiang Province (GC12B306) and the Program of Science and Technology Innovation Team in Heilongjiang (2012 TD 006).
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Chen, W., Yan, L., Gao, Y. et al. The removal characteristics and diversity of a microbial community capable of ammonia removal from compost. Ann Microbiol 66, 635–642 (2016). https://doi.org/10.1007/s13213-015-1141-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13213-015-1141-y