Abstract
Synthesis of cell-bound glucan from dietary sucrose by oral pathogenic bacteria may influence bacterial cell surface properties and colonization of surfaces. This study investigated the effects of the addition of 2 % sucrose to culture medium on cell surface properties (hydrophobicity, charge, and auto-aggregation) and colonization activities (attachment and biofilm formation) on three abiotic surfaces (hydroxyapatite, glass, and stainless steel) of two Streptococcus mutans strains, one Streptococcus salivarius strain, one Streptococcus mitis strain, and one Actinomyces naeslundii strain. The results showed that the additional sucrose reduced the hydrophobicity of three strains (44-62 %) and increased that of one strain (31 %). Cellular aggregation of one strain was decreased (13 %) and that of another increased (21 %). No change in the surface charge of strains was apparent. Additional sucrose also inhibited the attachment of three strains (0.6-1.3 log CFU cm−2) and enhanced that of one strain (0.5-1.3 log CFU cm−2) to glass and stainless steel. The attachment of two strains to hydroxyapatite was reduced (0.9-1.3 log CFU cm−2). Biofilm formation by four strains was enhanced on all surfaces (0.4-1 log CFU cm−2). No relationship between changes in cell surface properties and changes in colonization activities was apparent. Sucrose does not always enhance oral bacterial colonization of abiotic surfaces.
References
Abbott A, Rutter PR, Berkeley RCW (1983) The influence of ionic strength, ph and a protein layer on the interaction between Streptococcus mutans and glass surfaces. J Gen Microbiol 129:439–445
Aires CP, Tabchoury CP, Del Bel Cury AA, Koo H, Cury JA (2006) Effect of sucrose concentration on dental biofilm formed in situ and on enamel demineralization. Caries Res 40:28–32
Antonio AG, Moraes RS, Perrone D, Maia LC, Santos KRN, Iório NLP, Farah A (2010) Species, roasting degree and decaffeination influence the antibacterial activity of coffee against Streptococcus mutans. Food Chem 118:782–788
Apella MC, Venegas SC, Rodenas LAG, Belsa MA, Morando PJ (2008) Synthetic hydroxyapatite as a surface model of dental enamel and dentine. J Argent Chem Soc 97:109–118
Bayoudh S, Othmane A, Mora L, Ouada HB (2009) Assessing bacterial adhesion using DLVO and XDLVO theories and the jet impingement technique. Colloid Surf B 73:1–9
Chia TWR, Nguyen VT, McMeekin T, Fegan N, Dykes GA (2011) Stochasticity of bacterial attachment and its predictability by the extended Derjaguin-Landau-Verwey-Overbeek theory. Appl Environ Microbiol 77:3757–3764
Clark WB, Gibbons RJ (1977) Influence of salivary components and extracellular polysaccharide synthesis from sucrose on the attachment of Streptococcus mutans 6715 to hydroxyapatite surfaces. Infect Immun 18:514–523
Coleman RM, Georg LK, Rozzell AR (1969) Actinomyces naeslundii as an agent of human actinomycosis. Appl Environ Microbiol 18:420–426
Cury JA, Rebelo MA, Del Bel Cury AA, Derbyshire MT, Tabchoury CP (2000) Biochemical composition and cariogenicity of dental plaque formed in the presence of sucrose or glucose and fructose. Caries Res 34:491–497
Ellen RP, Balcerzak-Raczkowski IB (1977) Interbacterial aggregation of Actinomyces naeslundii and dental plaque streptococci. J Periodontal Res 12:11–20
Gibbons RJ, Etherden I (1983) Comparative hydrophobicities of oral bacteria and their adherence to salivary pellicles. Infect Immun 41:1190–1196
Hamada S, Tai S, Slade HD (1978) Binding of glucosyltransferase and glucan synthesis by Streptococcus mutans and other bacteria. Infect Immun 21:213–220
Jordan HV, Keyes PH (1966) In vitro methods for the study of plaque formation and carious lesions. Arch Oral Biol 11:793–802
Kolenbrander PE, London J (1993) Adhere today, here tomorrow: oral bacterial adherence. J Bacteriol 175:3247–3252
Lynch DJ, Fountain TL, Mazurkiewicz JE, Banas JA (2007) Glucan-binding proteins are essential for shaping Streptococcus mutans biofilm architecture. FEMS Microbiol Lett 268:158–165
Marshall KC, Stout R, Mitchell R (1971) Mechanism of the initial events in the sorption of marine bacteria to surfaces. J Gen Microbiol 68:337–348
Medilanski E, Kaufmann K, Wick LY, Wanner O, Harms H (2002) Influence of the surface topography of stainless steel on bacterial adhesion. Biofouling 18:193–203
Mukasa H, Slade HD (1973) Mechanism of adherence of Streptococcus mutans to smooth surfaces I. roles of insoluble dextran-levan synthetase enzymes and cell wall polysaccharide antigen in plaque formation. Infect Immun 8:555–562
Ohshima H (2009) Theory of electrostatics and electrokinetics of soft particles. Sci Technol Adv Mater 10:063001
Ozek Y (2011) Characterization of a large amount of water-insoluble polysaccharide synthesis Streptococcus salivarius. Int J Oral-Med Sci 10:25–30
Paes Leme AF, Koo H, Bellato CM, Bedi G, Cury JA (2006) The role of sucrose in cariogenic dental biofilm formation-new insight. J Dent Res 85:878–887
Rosenberg M, Gutnick D, Rosenberg E (1980) Adherence of bacteria to hydrocarbons: a simple method for measuring cell surface hydrophobicity. FEMS Microbiol Lett 9:29–33
Sato S, Yoshimitsu-Narita A, Eifuku H, Inoue M (1990) Isolation and some properties of extracellular glucan-producing strains of human oral Streptococcus salivarius. Microbios 62:101–112
Sherman JM, Niven CF Jr, Smiley KL (1943) Streptococcus salivarius and other non-hemolytic streptococci of the human throat. J Bacteriol 45:249–263
Slade HD (1976) In vitro models for the study of adherence of oral streptococci. In: Bowen WH, Genco RJ, O’Brien TC (eds) Immunologic aspects of dental caries, a special supplement to immunology abstracts. Information Retrieval, Inc., Washington, D.C., pp 21–28
Van Oss CJ, Good RJ, Chaudhury MK (1986) The role of van der Waals forces and hydrogen bonds in ‘hydrophobic interactions’ between biopolymers and low energy surfaces. J Colloid Interface Sci 111:378–390
Vickerman MM, Jones GW (1995) Sucrose-dependent accumulation of oral streptococci and their adhesion-defective mutants on saliva-coated hydroxyapatite. Oral Microbiol Immunol 10:175–182
Wang Y, Lee SM, Dykes GA (2013) Potential mechanism of the effects of tea extracts on the attachment, biofilm formation and cell size of Streptococcus mutans. Biofouling 29:307–318
Weerkamp AH, Jacobs T (1982) Cell wall-associated protein antigens of Streptococcus salivarius: purification, properties, and function in adherence. Infect Immun 38:233–242
Zero DT, van Houte J, Russo J (1986) The intra-oral effect on enamel demineralization of extracellular matrix material synthesized from sucrose by Streptococcus mutans. J Dent Res 65:918–923
Acknowledgements
The authors acknowledge financial support for this study from Monash University, Malaysia.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Wang, Y., Lee, S.M. & Dykes, G.A. Growth in the presence of sucrose may decrease attachment of some oral bacteria to abiotic surfaces. Ann Microbiol 65, 1159–1163 (2015). https://doi.org/10.1007/s13213-014-0883-2
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s13213-014-0883-2