Abstract
A mixture of carob pods and wheat bran as filler was evaluated as substrates for bioethanol production using Zymomonas mobilis in a 0.5 L-glass packed bed column fermenter. The experiments were initially performed under the optimum conditions defined previously in a flask-scale fermentation system. Under these conditions, removal of the generated heat and CO2 from the solid medium was more challenging than in the flask-scale system. In addition, the traditional aeration method was not applicable for removing the heat and CO2 since bioethanol production should be conducted under anaerobic conditions. The packed bed column system was modified, and an intermittent aeration method was used to remove the entrapped CO2 from the solid bed, thereby enabling the anaerobic condition to be maintained. The effect of operating temperature and carob particle size on the amount and rate of ethanol produced was also studied. We determined that the optimum operating conditions for this packed bed column fermentation system were 28 °C, a carob particle size of 1 mm, and 0.1 L min−1 intermittent aeration for 15 min each hour during the second 15 h of the fermentation process. Under these conditions 60.9 g ethanol per kilogram dry mixture of carob pod and wheat bran was produced.
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References
Amin G, Khalaf-Allah AM (1992) By-products formed during direct conversion of sugar beets to ethanol by Zymomonas mobilis in conventional submerged and solid-state fermentations. Biotechnol Lett 14:1187–1192
Balat M, Balat H (2009) Recent trends in global production and utilization of bio-ethanol fuel. Appl Energy 86:2273–2282
Biner B, Gubbuk H, Karhan M, Aksu M, Pekmezci M (2007) Sugar profiles of the pods of cultivated and wild types of carob bean (Ceratonia siliqua L.) in Turkey. Food Chem 100:1453–1455
Börjesson P (2009) Good or bad bioethanol from a greenhouse gas perspective—What determines this? Appl Energy 86:589–594
Caputi A Jr, Ueda M (1968) Spectrophotometric determination of ethanol in wine. Am J Enol Vitic 19:160–165
Figueroa-Montero A, Esparza-Isunza T, Saucedo-Castaneda G, Huerta-Ochoa S, Gutierrez-Rojasa M, Favela-Torresa E (2011) Improvement of heat removal in solid-state fermentation tray bioreactors by forced air convection. J Chem Technol Biotechnol 86:1321–1331
He M-X, Li Y, Liu Xn, Bai F, Feng H, Zhang Y-Z (2009) Ethanol production by mixed cultures of Paenibacillus sp. and Zymomonas mobilis using the raw starchy material from sweet potato. Ann Microbiol 59:749–754
Kwon YJ, Wang F, Liu CZ (2011) Deep-bed solid state fermentation of sweet sorghum stalk to ethanol by thermotolerant Issatchenkia orientalis IPE 100. Bioresour Technol 102:11262–11265
Li S, Li G, Zhang L, Zhou Z, Han B, Hou W, Wang J, Li T (2013) A demonstration study of ethanol production from sweet sorghum stems with advanced solid state fermentation technology. Appl Energy 102:260–265
Mazaheri D, Shojaosadati SA, Mousavi SM, Hejazi P, Saharkhiz S (2012) Bioethanol production from carob pods by solid-state fermentation with Zymomonas mobilis. Appl Energy 99:372–378
Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428
Mohanty SK, Behera S, Swain MR, Ray RC (2009) Bioethanol production from mahula (Madhuca latifolia L.) flowers by solid-state fermentation. Appl Energy 86:640–644
Nigam PS, Singh A (2011) Production of liquid biofuels from renewable resources. Prog Energy Combust Sci 37:52–68
Ogbonna CN, Okoli EC (2010) Conversion of cassava flour to fuel ethanol by sequential solid state and submerged cultures. Process Biochem 45:1196–1200
Pandey A, Larroche C, Soccol CR (2008) General considerations about solid-state fermentation processes. In: Pandey A, Soccol CR, Larroche C (eds) Current developments in solid-state fermentation. Springer Science, New Delhi, pp 13–25
Rodriguez LA, Toro ME, Vazquez F, Correa-Daneri ML, Gouiric SC, Vallejo MD (2010) Bioethanol production from grape and sugar beet pomaces by solid-state fermentation. Int J Hydrog Energy 35:5914–5917
Roukas T (1994a) Continuous ethanol-production from carob pod extract by immobilized Saccharomyces cerevisiae in a packed bed reactor. J Chem Technol Biotechnol 59:387–393
Roukas T (1994b) Solid-state fermentation of carob pods for ethanol production. Appl Microbiol Biotechnol 41:296–301
Saharkhiz S, Mazaheri D, Shojaosadati SA (2013) Evaluation of bioethanol production from carob pods by Zymomonas mobilis and Saccharomyces cerevisiae in solid-submerged fermentation. Prep Biochem Biotechnol 43:415–430
Sánchez S, Lozano LJ, Godínez C, Juan D, Pérez A, Hernández FJ (2010) Carob pod as a feedstock for the production of bioethanol in Mediterranean areas. Appl Energy 87:3417–3424
Sánchez-Segado S, Lozano LJ, Ríos AP, Hernández-Fernández FJ, Godínez C, Juan D (2012) Process design and economic analysis of a hypothetical bioethanol production plant using carob pod as feedstock. Bioresour Technol 104:324–328
Singhania RR, Patel AK, Soccol CR, Pandey A (2009) Recent advances in solid-state fermentation. Biochem Eng J 44:13–18
Turhan I, Bialka KL, Demirci A, Karhan M (2010) Ethanol production from carob extract by using Saccharomyces cerevisiae. Bioresour Technol 101:5290–5296
Vaheed H, Shojaosadati SA, Galip H (2011) Evaluation and optimization of ethanol production from carob pod extract by Zymomonas mobilis using response surface methodology. J Ind Microbiol Biotechnol 38:101–111
Yang S, Tschaplinski TJ, Engle NL, Carroll SL, Martin SL, Davison BH, Palumbo AV MR Jr, Brown SD (2009) Transcriptomic and metabolomic profiling of Zymomonas mobilis during aerobic and anaerobic fermentations. BMC Genomics 10:34
Yu J, Zhang X, Tan T (2008) Ethanol production by solid state fermentation of sweet sorghum using thermotolerant yeast strain. Fuel Process Technol 89:1056–1059
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Mazaheri, D., Shojaosadati, S.A., Hejazi, P. et al. Bioethanol production performance in a packed bed solid-state fermenter: evaluation of operational factors and intermittent aeration strategies. Ann Microbiol 65, 351–357 (2015). https://doi.org/10.1007/s13213-014-0867-2
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DOI: https://doi.org/10.1007/s13213-014-0867-2