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Promoting plant growth in a commercial rice cultivar by endophytic diazotrophic bacteria isolated from rice landraces

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Abstract

Population density of endophytic diazotrophic bacteria (EDB) was highest in the rice landrace root tissues at nursery stage. Indole-3-acetic acid (IAA) production (0.85–16.66 μg mL−1) was found in 21 strains tested. More than 80 % (18 isolates) of the isolates solubilized phosphate, while only 28.57 % (six isolates) of selected strains produced siderophore. Seventy-one percent of tested isolates produced ammonia. The effects of EDB isolated from rice landraces on seed and on the growth of the commercial jasmine rice cultivar Khao Dawk Mali 105 were evaluated in greenhouse. Inoculation of all EDB on rice seeds significantly increased nitrogen content in roots (P = 0.05). The potentially useful isolates belonged to four different genera Burkholderia, Klebsiella, Novosphingobium and Sphingomonas. In vivo colonization of Burkholderia sp. SS5, Klebsiella sp. SS2, Novosphingobium sp. TR4 and Sphingomonas sp. PS5 was confirmed using the commercial rice cultivar Khao Dawk Mali 105 as a model host. The inoculated roots with ß-glucuronidase (GUS)-tagged bacteria exhibited a blue color, which was most intense at the tip of root hairs, root tips, germination point and leaf tips.

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Acknowledgments

This research was supported by Office of the Higher Education Commission, Thailand under the National Research University Project and The Thailand Research Fund (Research-Team Promotion Grant RTA5580007). We thank The Graduated School of Chiang Mai University, Thailand for support, as well as The Model Farm Project, the Royal Initiatives of Her Majesty the Queen, Khun Tae Village, Thailand for assistance in sampling collection.

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  1. Microbiology Division, Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, 50200, Thailand

    Chakrapong Rangjaroen & Saisamorn Lumyong

  2. Lanna Rice Research Centre, Faculty of Agriculture, Chiang Mai University, Chiang Mai, 50200, Thailand

    Benjavan Rerkasem

  3. School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Nakhonratchasima, 30000, Thailand

    Neung Teaumroong & Rujirek Noisangiam

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  1. Chakrapong Rangjaroen
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Rangjaroen, C., Rerkasem, B., Teaumroong, N. et al. Promoting plant growth in a commercial rice cultivar by endophytic diazotrophic bacteria isolated from rice landraces. Ann Microbiol 65, 253–266 (2015). https://doi.org/10.1007/s13213-014-0857-4

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