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Lipase production by Serratia marcescens strain SN5gR isolated from the scat of lion-tailed macaque (Macaca silenus) in Silent Valley National Park, a biodiversity hotspot in India

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Abstract

An extracellular lipase-producing bacterium was isolated from a fecal sample of lion-tailed macaque (Macaca silenus), an endangered Old World monkey that is endemic to the Western Ghats of South India. Morphological, biochemical and molecular analyses identified the bacterium as Serratia marcescens. Production of lipase was investigated in shake-flask culture. Optimum tributyrin concentration of 1.5 % was found to be the most suitable triglyceride to increase lipase production (13.3 U ml−1). The next best lipid source observed was olive oil (11.94 U ml−1), followed by castor oil, coconut oil and palm oil. Analyzing the effect of different carbon sources on lipase production revealed that 2 % glucose yielded higher lipase production than the other tested carbon sources. Investigations on suitable nitrogen source for lipase production revealed that 2 % meat extract yielded higher lipase production. The most suitable trace element for maximum lipase production was zinc sulfate, followed by magnesium sulfate and copper sulfate. Partial characterization of the crude lipase revealed that pH 7.0 and a temperature of 40 °C gave optimal lipase activity. Enzymatic activity of the crude sample was retained over a wide temperature range (20–75 °C), and 70 % of enzyme activity was retained at 60 °C. Testing the effect of various organic solvents on lipase activity revealed that hexadecane increased lipase activity by 85 % over the control.

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Acknowledgments

B.G., S.M. and K.G. acknowledge the support of technical facilities available in Presidency University and Bethune College (Govt. of West Bengal, India) respectively. B.G., K.G. and S.M. also acknowledge the financial assistance from the University Grants Commission (Govt. of India) for carrying out this research program. The authors also wish to express their sincere regards to the anonymous referees and the editorial board for the helpful comments to improve the manuscript of the research paper.

Competing interests

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Molecular Biology Laboratory, Department of Biotechnology, Presidency University, 86/1 College Street, Kolkata, 700073, India

    Bhaskar Gupta

  2. Department of Botany, Bethune College, 181 Bidhan Sarani, Kolkata, 700006, India

    Kamala Gupta

  3. Department of Zoology and Molecular Biology & Genetics, Presidency University, 86/1 College Street, Kolkata, 700073, India

    Souryadeep Mukherjee

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  1. Bhaskar Gupta
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Correspondence to Souryadeep Mukherjee.

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Gupta, B., Gupta, K. & Mukherjee, S. Lipase production by Serratia marcescens strain SN5gR isolated from the scat of lion-tailed macaque (Macaca silenus) in Silent Valley National Park, a biodiversity hotspot in India. Ann Microbiol 63, 649–659 (2013). https://doi.org/10.1007/s13213-012-0515-7

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  • DOI: https://doi.org/10.1007/s13213-012-0515-7

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