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Detection of Salmonella typhimurium by antibody/enzyme-conjugated magnetic nanoparticles

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Abstract

A simple, highly sensitive method was developed to detect Salmonella typhimurium using 100-nm antibody- and horseradish peroxidase-conjugated magnetic nanoparticles (Ab-HRP-MNPs). The particles bound bacteria, while unbound particles were removed by filtration. The number of bacteria was determined based on the intensity of light emitted from the chemiluminescence reaction catalysed by bacterium-bound peroxidase in the filter. The light intensity was proportional to the cells in the range of 0–104 colony forming units (CFU), with a lower detection limit of 10 CFU.

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Authors and Affiliations

  1. Amogreentech Co., Ltd. Gangneung Science Industry Park, Block GA-13-1 #216, Gangneung, 210-340, Korea

    Saem Mun

  2. Department of Chemistry, Gangneung-Wonju National University, Gangneung, 210-702, Korea

    Suk-Jung Choi

Authors
  1. Saem Mun
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  2. Suk-Jung Choi
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Correspondence to Suk-Jung Choi.

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Mun, S., Choi, SJ. Detection of Salmonella typhimurium by antibody/enzyme-conjugated magnetic nanoparticles. BioChip J 9, 10–15 (2015). https://doi.org/10.1007/s13206-014-9102-2

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  • DOI: https://doi.org/10.1007/s13206-014-9102-2

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