Abstract
In this study, a microfluidics is integrated with a laser to develop a microfluidic system for single-cell capture and cellular DNA denaturation. The results show that the cells encountered low hydrodynamic stress at low velocities and, thus, could be captured by a cell capture structure. Laser heating of the solution at low velocity produced bubbles that pushed the cells away from the capturing areas. The released cells were subsequently lysed, and cellular DNA was extended and immobilized on the surface of pillars before undergoing laser heating to induce partial denaturation.
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Hung, MS., Huang, YT. Laser-induced heating for cell release and cellular DNA denaturation in a microfluidics. BioChip J 7, 319–324 (2013). https://doi.org/10.1007/s13206-013-7402-6
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DOI: https://doi.org/10.1007/s13206-013-7402-6