Original Article

3 Biotech

, Volume 1, Issue 2, pp 91-97

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

New microsatellite markers for assessment of genetic diversity in date palm (Phoenix dactylifera L.)

  • Khaled ElmeerAffiliated withBiotechnology Centre, Ministry of Environment Email author 
  • , Hina SarwathAffiliated withBiotechnology Centre, Ministry of Environment
  • , Joel MalekAffiliated withWeill Cornell Medical College in Qatar
  • , Michael BaumAffiliated withInternational Center for Agricultural Research in the Dry Areas (ICARDA)
  • , Aladdin HamwiehAffiliated withInternational Center for Agricultural Research in the Dry Areas (ICARDA)


New primer pairs of genomic DNA microsatellite markers were tested to assess the genetic diversity of eleven date palm genotypes. The results indicated that out of thirty, only seven primers (23.3%) failed to amplify the expected PCR fragments, while thirteen primers (43.3%) amplified monomorphic banding patterns and the remaining ten primers (33.4%) generated polymorphic banding patterns. A total of 77 alleles have been observed with a mean of 7.7 alleles per locus. The average of gene diversity was 0.80 ranging from 0.6 (in marker DP168) to 0.9 (in two markers DP157 and DP175). These new co-dominant markers will be a starting point for researchers making use of the markers for genetic mapping and diversity analysis of date palm.


Phoenix dactylifera Microsatellite marker Simple sequence repeats