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Beneficial effects of fermented sardinelle protein hydrolysates on hypercaloric diet induced hyperglycemia, oxidative stress and deterioration of kidney function in wistar rats

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Abstract

This study investigated the potential effects of fermented sardinelle protein hydrolysates (FSPHs) obtained by two proteolytic bacteria, Bacillus subtilis A26 (FSPH-A26) and Bacillus amyloliquefaciens An6 (FSPH-An6), on hypercaloric diet (HCD) induced hyperglycemia and oxidative stress in rats. Effects of FSPHs on blood glucose level, glucose tolerance, α-amylase activity and hepatic glycogen content were investigated, as well as their effect on the oxidative stress state. Biochemical findings revealed that, while undigested sardinelle proteins did not exhibit hypoglycemic activity, oral administration of FSPHs to HCD-fed rats reduced significantly α-amylase activity as well as glycemia and hepatic glycogen levels. Further, the treatment with FSPHs improved the redox status by decreasing the levels of lipid peroxidation products and increasing the activities of the antioxidant enzymes (superoxide dismutase, glutathione peroxidase and catalase) and the level of glutathione in the liver and kidneys, as compared to those of HCD-fed rats. FSPHs were also found to exert significant protective effects on liver and kidney functions, evidenced by a marked decrease in alkaline phosphatase activity and a modulation of creatinine and uric acid contents. These results indicated the beneficial effect of FSPHs on the prevention from hyperglycemia and oxidative stress.

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Acknowledgements

This work was funded by the Ministry of Higher Education and Scientific Research-Tunisia. Emerging Research Group Grant from Generalitat Valenciana in Spain (GV/2015/138) and JAEDOC-CSIC postdoctoral contract of L.M. cofounded by the European Social Found are acknowledged.

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Authors and Affiliations

  1. Laboratory of Enzyme Engineering and Microbiology, National School of Engineering of Sfax (ENIS), University of Sfax, P. O. Box 1173, 3038, Sfax, Tunisia

    Ines Jemil, Rim Nasri, Ola Abdelhedi, Rabeb Ben Slama-Ben Salem, Mohamed Hajji & Moncef Nasri

  2. Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax (FSS), University of Sfax, P. O. Box 95, 3052, Sfax, Tunisia

    Abdelfattah ElFeki

  3. Laboratory of Hematology, Habib Bourguiba Hospital, 3029, Sfax, Tunisia

    Choumous Kallel

  4. Laboratory of Biochemistry, CHU Hedi Chaker, 3029, Sfax, Tunisia

    Rim Marrekchi & Kamel Jamoussi

  5. Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, 46980, Valencia, Spain

    Maria-Concepción Aristoy & Fidel Toldrá

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  1. Ines Jemil
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  2. Rim Nasri
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  3. Ola Abdelhedi
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  4. Maria-Concepción Aristoy
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  5. Rabeb Ben Slama-Ben Salem
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  6. Choumous Kallel
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  7. Rim Marrekchi
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  8. Kamel Jamoussi
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  9. Abdelfattah ElFeki
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  10. Mohamed Hajji
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  11. Fidel Toldrá
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  12. Moncef Nasri
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Correspondence to Ines Jemil.

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Jemil, I., Nasri, R., Abdelhedi, O. et al. Beneficial effects of fermented sardinelle protein hydrolysates on hypercaloric diet induced hyperglycemia, oxidative stress and deterioration of kidney function in wistar rats. J Food Sci Technol 54, 313–325 (2017). https://doi.org/10.1007/s13197-016-2464-9

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  • DOI: https://doi.org/10.1007/s13197-016-2464-9

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