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The effect of ultraviolet treatment on enzymatic activity and total phenolic content of minimally processed potato slices

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Abstract

In this work, potato slices were exposed to different doses of UV-C irradiation (i.e. 2.28, 6.84, 11.41, and 13.68 kJ m−2) with or without pretreatment [i.e. ascorbic acid and calcium chloride (AACCl) dip] and stored at 4 ± 1 °C. Changes in enzymatic activities of polyphenol oxidase (PPO), peroxidase (POD) and phenylalanine ammonia lyase (PAL), as well as total phenolic content (TPC) were investigated after 0, 3, 7 and 10 days of storage. Results showed that untreated and UV-C treated potato slices at 13.68 kJ m−2 dosage level showed significantly higher PPO, POD and PAL activities. Conversely, untreated potato slices showed the lowest TPC during storage period. Potato slices subjected to AACCl dip plus UV-C at 6.84 kJ m−2 produced lower PPO, POD and PAL activities, as well as maintained a high TPC during storage.

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References

  • Ahvenaine R (1996) New approaches in improving the shelf life of minimally processed fruit and vegetables. Trends Food Sci Technol 7:179–187

    Article  Google Scholar 

  • Akanbi CT, Gureje PO, Adeyemi IA (1996) Effect of heat moisture pre-treatment on physical characteristics of dehydrated yam. J Food Eng 28:45–54

    Article  Google Scholar 

  • Alegria C, Pinheiro J, Duthoit M, Gonçalves EM, Moldão-Martins M, Abreu M (2012) Fresh-cut carrot (cv. Nantes) quality as affected by abiotic stress (heat shock and UV-C irradiation) pre-treatments. LWT Food Sci Technol 48:197–203

    Article  CAS  Google Scholar 

  • Artés-Hernández F, Robles PA, Gómez PA, Tomás-Callejas A, Artés F (2010) Low UV-C illumination for keeping overall quality of fresh-cut watermelon. Postharvest Biol Technol 55:114–120

    Article  Google Scholar 

  • Basaran N, Quintero-Ramos A, Moake MM, Churey JJ, Worobo RW (2004) Influence of apple cultivars on inactivation of different strains of Escherichia coli O157:H7 in apple cider by UV irradiation. Appl Environ Microbiol 70:6061–6065

    Article  CAS  Google Scholar 

  • Ben-Yehoshua S, Rodov V, Kim JJ, Carmeli S (1992) Preformed and induced antifungal materials of citrus fruits in relation to the enhancement of decay resistance by heat and ultraviolet treatments. J Agric Food Chem 40:1217–1221

    Article  CAS  Google Scholar 

  • Bett-Garber KL, Lamikanra O, Lester GE, Ingram DA, Watson MA (2005) Influence of soil type and storage conditions on sensory qualities of fresh-cut cantaloupe (Cucumis melo). J Sci Food Agric 85:825–830

    Article  CAS  Google Scholar 

  • Cabezas-Serrano AB, Amodio ML, Cornacchia R, Rinaldi R, Colelli G (2009) Suitability of five different potato cultivars (Solanum tuberosum L.) to be processed as fresh-cut products. Postharvest Biol Technol 53:138–144

    Article  CAS  Google Scholar 

  • Cantos E, Espín JC, Tomás-Barberán FA (2001a) Effect of wounding on phenolic enzymes in six minimally processed lettuce cultivars upon storage. J Agric Food Chem 49:322–330

    Article  CAS  Google Scholar 

  • Cantos E, Espín JC, Tomás-Barberán FA (2001b) Postharvest induction modeling method using UV irradiation pulses for obtaining resveratrol-enriched table grapes: a new “functional” fruit? J Agric Food Chem 49:5052–5058

    Article  CAS  Google Scholar 

  • Cantos E, Tudela JA, Gil MI, Espín JC (2002) Phenolic compounds and related enzymes are not rate-limiting in browning development of fresh-cut potatoes. J Agric Food Chem 50:3015–3023

    Article  CAS  Google Scholar 

  • Cheynier V (2005) Polyphenols in foods are more complex than often thought. Am J Clin Nutr 81:223S–229S

    CAS  Google Scholar 

  • Chiavaro E, Barbanti D, Vittadini E, Massini R (2006) The effect of different cooking methods on the instrumental quality of potatoes (cv. Agata). J Food Eng 77:169–178

    Article  Google Scholar 

  • Crupi P, Pichierri A, Basile T, Antonacci D (2013) Postharvest stilbenes and flavonoids enrichment of table grape cv Redglobe (Vitis vinifera L.) as affected by interactive UV-C exposure and storage conditions. Food Chem 141:802–808

    Article  CAS  Google Scholar 

  • Degl’Innocenti E, Guidi L, Pardossi A, Tognoni F (2005) Biochemical study of leaf browning in minimally processed leaves of lettuce (Lactuca sativa L. var. Acephala). J Agric Food Chem 53:9980–9984

    Article  Google Scholar 

  • Ding P, Yap SL (2014) Browning assessment methods and polyphenol oxidase in UV-C irradiated Berangan banana fruit. Int Food Res J 21:1667–1674

    CAS  Google Scholar 

  • Erkan M, Wang CY, Krizek DT (2001) UV-C irradiation reduces microbial populations and deterioration in Cucurbitapepo fruit tissue. Environ Exp Bot 45:1–9

    Article  Google Scholar 

  • Geveke DJ (2008) UV inactivation of E. coli in liquid egg white. Food Bioprocess Technol 1:201–206

    Article  Google Scholar 

  • Gil MI, Martí JA, Artés F (1996) Minimally processed pomegranate seeds. LWT Food Sci Technol 29:708–713

    Article  CAS  Google Scholar 

  • Gómez PL, Alzamora SM, Castro MA, Salvatori DM (2010) Effect of ultraviolet-C light dose on quality of cut-apple: microorganism, color and compression behavior. J Food Eng 98:60–70

    Article  Google Scholar 

  • Hiraga S, Sasaki K, Ito H, Ohashi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468

    Article  CAS  Google Scholar 

  • Ibarz A, Garvín A, Garza S, Pagán J (2009) Inactivation of carboxypeptidase A and trypsin by UV–visible light. Innov Food Sci Emerg Technol 10:517–521

    Article  CAS  Google Scholar 

  • Jang JH, Moon KD (2011) Inhibition of polyphenol oxidase and peroxidase activities on fresh cut apple by simultaneous treatment of ultrasound and ascorbic acid. Food Chem 124:444–449

    Article  CAS  Google Scholar 

  • Jones DH (1984) Phenylalanine ammonia-lyase: regulation of its induction, and its role in plant development. Phytochemistry 23:1349–1359

    Article  CAS  Google Scholar 

  • Kahn V (1977) Some biochemical properties of polyphenoloxidase from two avocado varieties differing in their browning rates. J Food Sci 42:38–43

    Article  CAS  Google Scholar 

  • Kang HM, Saltveit ME (2003) Wound-induced increases in phenolic content of fresh-cut lettuce is reduced by a short immersion in aqueous hypertonic solutions. Postharvest Biol Technol 29:271–277

    Article  CAS  Google Scholar 

  • Koutchma T (2009) Advances in ultraviolet light technology for non-thermal processing of liquid foods. Food Bioprocess Technol 2:138–155

    Article  CAS  Google Scholar 

  • Koutchma T, Forney LJ, Moraru CI (2009) Ultraviolet light in food technology: principles and applications. CRC Press, Boca Raton, USA, pp 1–278

  • Lamikanra G (2002) Enzymatic effects on flavor and texture of fresh-cut fruits and vegetables. In: Lamikanra G (ed) Fresh-cut fruits and vegetables. Science, technology and market. CRC Press, LLC, Boca Raton, pp 125–185

    Chapter  Google Scholar 

  • Lamikanra G, Kueneman D, Ukuku D, Bett-Garber KL (2005) Effect of processing under ultraviolet light on the shelf life of fresh-cut cantaloupe melon. J Food Sci 70:C534–C539

    Article  CAS  Google Scholar 

  • Lasekan O, Lasekan W, Idowu M, Ojo O (1996) Effect of extrusion cooking conditions on the nutritional value, storage stability and sensory characteristics of a maize-based snack food. J Cereal Sci 24:79–85

    Article  Google Scholar 

  • López-Rubira V, Conesa A, Allende A, Artés F (2005) Shelf life and overall quality of minimally processed pomegranate arils modified atmosphere packaged and treated with UV-C. Postharvest Biol Technol 37:174–185

    Article  Google Scholar 

  • MacAdam JW, Nelson CJ, Sharp RE (1992) Peroxidase activity in the leaf elongation zone of tall fescue I. Spatial distribution of ionically bound peroxidase activity in genotypes differing in length of the elongation zone. Plant Phy 99:872–878

    Article  CAS  Google Scholar 

  • Maghoumi M, Gómez PA, Mostofi Y, Zamani Z, Artés-Hernández F, Artés F (2013a) Combined effect of heat treatment, UV-C and superatmospheric oxygen packing on phenolics and browning related enzymes of fresh-cut pomegranate arils. LWT Food Sci Technol 54:389–396

    Article  CAS  Google Scholar 

  • Maghoumi M, Gómez PA, Artés-Hernández F, Mostofi Y, Zamani Z, Artés F (2013b) Hot water, UV-C and superatmospheric oxygen packaging as hurdle techniques for maintaining overall quality of fresh-cut pomegranate arils. J Sci Food Agric 93:1162–1168

    Article  CAS  Google Scholar 

  • Marangoni AG, Palma T, Stanley DW (1996) Membrane effects in postharvest physiology. Postharvest Biol Technol 7:193–217

    Article  Google Scholar 

  • Matak KE, Churey JJ, Worobo RW, Sumner SS, Hovingh E, Hackney CR (2004) Efficacy of UV light for the reduction of Listeria monocytogenes in goat’s milk. J Food Prot 68:2212–2216

    Google Scholar 

  • Murata M, Tanaka E, Minoura E, Homma S (2004) Quality of cut lettuce treated by heat shock: prevention of enzymatic browning, repression of phenylalanine ammonia lyase activity, and improvement on sensory evaluation during storage. Biosci Biotechnol Biochem 68:501–507

    Article  CAS  Google Scholar 

  • Ohlsson T (1994) Minimally processing—preservation methods of the future: an overview. Trends Food Sci Technol 5:341–344

    Article  CAS  Google Scholar 

  • Ojeda GA, Sgroppo SC, Zaritzky NE (2014) Application of edible coatings in minimally processed sweet potatoes (Ipomoea batatas L.) to prevent enzymatic browning. Int J Food Sci Technol 49:876–883

    Article  CAS  Google Scholar 

  • Olivas GI, Mattinson DS, Barbosa-Cánovas GV (2007) Alginate coatings for preservation of minimally processed ‘Gala’apples. Postharvest Biol Technol 45:89–96

    Article  CAS  Google Scholar 

  • Onwueme IC, Charles WB (1994) Tropical root and tuber crops: production, perspectives and future prospects. Food & Agriculture Org, Rome, Italy, pp 92–98

  • Oteiza JM, Peltzer M, Gannuzzi L, Zaritzky N (2005) Antimicrobial efficacy of UV radiation on Escherichia coli O157:H7 (EDL 933) in fruit juices of different absorptivities. J Food Prot 68:49–58

    Google Scholar 

  • Ozo ON, Caygill JC, Coursey DG (1984) Phenolics of five yam (Dioscorea) species. Phytochemistry 23:329–331

    Article  CAS  Google Scholar 

  • Parkin KL, Marangoni A, Jackman RL, Yada RY, Stanley DW (1989) Chilling injury. A review of possible mechanisms. J Food Biochem 13:127–153

    Article  CAS  Google Scholar 

  • Richard-Forget FC, Gauillard FA (1997) Oxidation of chlorogenic acid, catechins, and 4 methylcatechol in model solutions by combinations of pear (Pyrus communis cv. Williams) polyphenol oxidase and peroxidase: a possible involvement of peroxidase in enzymatic browning. J Agric Food Chem 45:2472–2476

    Article  CAS  Google Scholar 

  • Robinson DS (1991) Peroxidases and catalases in foods. In: Robinson DS (ed) Oxidative enzymes in foods. Elsevier Applied Science, London, pp 1–47

  • Rocha A, Morais AM (2002) Polyphenoloxidase activity and total phenolic content as related to browning of minimally processed ‘Jonagored’apple. J Sci Food Agric 82:120–126

    Article  CAS  Google Scholar 

  • Saltveit ME (1996) Physical and physiological changes in minimally processed fruits and vegetables. In: Proceedings-Phytochemical Society of Europe, vol 41. Oxford University Press Inc., Oxford, pp 205–220

  • Saltveit ME (2000) Wound induced changes in phenolic metabolism and tissue browning are altered by heat shock. Postharvest Biol Technol 21:61–69

    Article  CAS  Google Scholar 

  • Selvarajah S, Herath HMW, Bandara DC (1998) Physiological effects of pre heat treatment on pineapple fruit stored at low temperatures. Trop Agric Res 10:417–419

    Google Scholar 

  • Severini C, Baiano A, De Pilli T, Romaniello R, Derossi A (2003) Prevention of enzymatic browning in sliced potatoes by blanching in boiling saline solutions. LWT Food Sci Technol 36:657–665

    Article  CAS  Google Scholar 

  • Shama G (2007) Process challenges in applying low doses of ultraviolet light to fresh produce for eliciting beneficial hormetic responses. Postharvest Biol Technol 44:1–8

    Article  CAS  Google Scholar 

  • Shen Y, Sun Y, Qiao L, Chen J, Liu D, Ye X (2013) Effect of UV-C treatments on phenolic compounds and antioxidant capacity of minimally processed Satsuma mandarin during refrigerated storage. Postharvest Biol Technol 76:50–57

    Article  CAS  Google Scholar 

  • Singleton VL, Rossi JA (1965) Colorimetry of total phenolics with phosphomolybdic phosphotungstic acid reagents. Am J Enol Vitic 16:144–158

    CAS  Google Scholar 

  • Stutte GW (1989) Quantification of net enzymatic activity in developing peach fruit using computer video image analysis. Hort Sci 24:113–115

    CAS  Google Scholar 

  • Sun J, Xiang X, Yu C, Shi J, Peng H, Yang B, Jiang Y (2009) Variations in contents of browning substrates and activities of some related enzymes during litchi fruit development. Sci Hortic 120:555–559

    Article  CAS  Google Scholar 

  • Toivonen PMA (1992) The reduction of browning in parsnips. J Hort Sci 67:547–551

    CAS  Google Scholar 

  • Tomás-Barberán FA, Espin JC (2001) Phenolic compounds and related enzymes as determinants of quality in fruits and vegetables. J Sci Food Agric 81:853–876

    Article  Google Scholar 

  • Vitti MCD, Sasaki FF, Miguel P, Kluge RA, Moretti CL (2011) Activity of enzymes associated with the enzymatic browning of minimally processed potatoes. Braz Arch Biol Technol 54:983–990

    Article  CAS  Google Scholar 

  • Walkling-Ribeiro M, Noci F, Cronin DA, Lyng JG, Morgan DJ (2009) Shelf life and sensory evaluation of orange juice after exposure to thermosonication and pulsed electric fields. Food Bioprod Process 87:102–107

    Article  Google Scholar 

  • Wang H, Peng H, Luo Y (2007) Control of browning and microbial growth on fresh-cut apples by sequential treatment of sanitizers and calcium ascorbate. J Food Sci 72:M1–M7

    Article  CAS  Google Scholar 

  • Wang Q, Cao Y, Zhou L, Jiang CZ, Feng Y, Wei S (2015) Effects of postharvest curing treatment on flesh colour and phenolic metabolism in fresh-cut potato products. Food Chem 169:246–254

    Article  CAS  Google Scholar 

  • Zhang Z, Pang X, Xuewu D, Ji Z, Jiang Y (2005) Role of peroxidase in anthocyanin degradation in litchi fruit pericarp. Food Chem 90:47–52

    Article  Google Scholar 

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Acknowledgments

The authors are grateful for the extensive financial support of the Fundamental Research Scheme (No. 5524558) at the University Putra Malaysia.

Author contributions

Li Shing Teoh was involved with planning, collecting, interpreting and drafting of the manuscript. Lasekan, Noranizan and Norhashila conceived the idea and edited the manuscript. All authors read and approved the final manuscript.

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Correspondence to Ola Lasekan.

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Teoh, L.S., Lasekan, O., Adzahan, N.M. et al. The effect of ultraviolet treatment on enzymatic activity and total phenolic content of minimally processed potato slices. J Food Sci Technol 53, 3035–3042 (2016). https://doi.org/10.1007/s13197-016-2275-z

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  • DOI: https://doi.org/10.1007/s13197-016-2275-z

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