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Antioxidant activity of three components of wheat leaves: ferulic acid, flavonoids and ascorbic acid

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Abstract

Recent studies have shown that wheat leaves are sources of natural antioxidant substances. In this study, the content of three antioxidants (ascorbic acid, total flavonoids and ferulic acid) and antioxidant activities of wheat leaf extracts were evaluated using 2,2-diphenyl-β-picrylhydrazyl and 2,2-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) assays. The interaction activities among different antioxidants were analyzed by factorial and path analyses. Wheat leaves were extracted with solvents of different polarities. The ABTS test showed that the ethanol extract had the highest antioxidant activity. There were highly significant linear correlations between antioxidant capacities of the investigated wheat leaves and their ascorbic acid, ferulic acid and flavonoid contents. Total flavonoids and ascorbic acid were major contributors to total antioxidant capacity in wheat leaves.

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References

  • Arora A, Sairam RK, Srivastave GC (2002) Oxidative stress and antioxidative systems in plants. Curr Sci 82:1227–1238

    CAS  Google Scholar 

  • Cervellati R, Renzulli C, Guerra MC, Speroni E (2002) Evaluation of antioxidant activity of some natural polyphenolic compounds using the Briggs-Rauscher reaction method. J Agric Food Chem 50:7504–7509

    Article  CAS  Google Scholar 

  • Chu Y, Sun J, Wu X, Liu R (2002) Antioxidant and antiproliferative activities of common vegetables. J Agric Food Chem 50:9610–9616

    Google Scholar 

  • Deng B, Jiang GB, Huang HY, Chen LP (2008) Determination of total flavones content in Prunella vulgaris L. by spectrophotometry. Lishizhen Med Mater Med Res 7:1608–1609

    Google Scholar 

  • Erlund I (2004) Review of the flavonoids quercetin, hesperetin, and naringenin. Dietary sources, bioactivities, bioavailability, and epidemiology. Nutr Res 24:851–874

    Article  CAS  Google Scholar 

  • Joanna K, Lucja F (2006) Ferulic acid and its position among the phenolic compounds of wheat. Crit Rev Food Sci Nutr 46:639–647

    Article  Google Scholar 

  • Galato D, Ckless K, Susin MF, Giacomelli C, Ribeiro-do-Valle RM, Spinelli A (2001) Antioxidant capacity of phenolic and related compounds: correlation among electrochemical, visible spectroscopy methods and structure-antioxidant activity. Redox Rep 6:243–250

    Article  CAS  Google Scholar 

  • Gutteridge JMC, Halliwell B (2000) Free radicals and antioxidants in the year 2000: a historical look to the future. Ann N Y Acad Sci 899:136–147

    Article  CAS  Google Scholar 

  • Kähkönen MP, Hopia AI, Heinonen M (2001) Berry phenolics and their antioxidant activity. J Agric Food Chem 49:4076–4082

    Article  Google Scholar 

  • Proteggente AR, Pannala AS, Paganga G, Van Buren L, Wager E, Wiseman S, Van DePut F, Dacombe C, Rice-Evans CA (2002) The antioxidant activity of regularly consumed fruit and vegetables reflects their phenolic and vitamin C composition. Free Radic Res 36:217–233

    Article  CAS  Google Scholar 

  • Omidreza F, Antonio L, Rita P, Giancarlo M, Luciano S (2005) Evaluation of the antioxidant activity of flavonoids by “ferric reducing antioxidant power” assay and cyclic voltammetry. Biochim Biophys Acta 1721:174–184

    Article  Google Scholar 

  • Raghu V, Kalpana P, Srinivasan K (2007) Comparison of ascorbic acid content of Emblica officinalis fruits determined by different analytical methods. J Food Compos Anal 20:529–533

    Article  CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26:1231–1237

    Article  CAS  Google Scholar 

  • Schlesier K, Harwat M, Bohm V, Bitsch R (2002) Assessment of antioxidant activity by using different in vitro methods. Free Radic Res 36:177–187

    Article  CAS  Google Scholar 

  • Sun T, Ho CT (2005) Antioxidant activities of buckwheat extracts. Food Chem 90:743–749

    Article  CAS  Google Scholar 

  • Tang SZ, Kerry JP, Sheehan D, Buckley DJ (2002) Antioxidative mechanisms of tea catechins in chicken meat systems. Food Chem 76:45–51

    Article  CAS  Google Scholar 

  • Tuanjai N, Supalax S, Thawatchai T, Wittaya N (2011) New approach for evaluation of the antioxidant capacity based on scavenging DPPH free radical in micelle systems. Food Res Int 44:798–806

    Article  Google Scholar 

  • Zhang ZQ, Wang X, Yao YY, Meng Z (2010) Determination of ferulic acid in wheat bran ultrasonic assisted alkaline-alcohol extraction and HPLC detection. J Chin Cereals Oils Assoc 4:89–92

    Google Scholar 

  • Zhou K, Laux JJ, Yu L (2004) Comparison of Swiss red wheat grain and fractions for their antioxidant properties. J Agric Food Chem 52:1118–1123

    Article  CAS  Google Scholar 

  • Zhou KQ, Yu LL (2004) Effects of extraction solvent on wheat bran antioxidant activity estimation. LWT Food Sci Technol 37:717–721

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This project is financially supported by the Key Scientific Fund (No. 09ZA081) administered by the Sichuan Education Committee of the People’s Republic of China.

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Correspondence to Zhi-Qing Zhang.

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Zhang, ZQ., Xiang, JJ. & Zhou, LM. Antioxidant activity of three components of wheat leaves: ferulic acid, flavonoids and ascorbic acid. J Food Sci Technol 52, 7297–7304 (2015). https://doi.org/10.1007/s13197-015-1805-4

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  • DOI: https://doi.org/10.1007/s13197-015-1805-4

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