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Characterization and in vitro biological activities of Thai traditional fermented shrimp pastes

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Abstract

In this work, chemical and biological characteristics of two types of Thai fermented shrimp paste, Kapi Ta Dam and Kapi Ta Deang, at different fermentation periods and their raw materials were investigated. Kapi had low water activity and high proteins with high glutamic acid and lysine. Both Kapis, which had different sources, showed similar characteristics. The number of lactic acid bacteria in the products increased during the early stages of fermentation. Free α-amino acid contents in the products increased with the fermentation time. The water extracts from Kapi products showed strong antioxidative activities against ABTS+ radical, and ACE inhibitory activity but they did not exhibit antimicrobial activity against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella Typhimurium. Biological activities in Kapi could be developed by fermentation process, enzymatic hydrolysis of proteins and non-enzymatic browning reactions. Kapi could, thus, serve as a potential source of natural bioactive substances.

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References

  • Adler-Nissen J (1979) Determination of the degree if hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J Agric Food Chem 27(6):1256–1262

    Article  CAS  Google Scholar 

  • AOAC (1995) Official methods of analysis, 16th edn. AOAC International, Virginia

    Google Scholar 

  • Binsan W, Benjakul S, Visessanguan W, Roytrakul S, Tanaka M, Kishimura H (2008) Antioxidative activity of Mungoong, an extract paste, from the cephalothorax of white shrimp (Litopenaeus vannamei). Food Chem 106(1):185–193

    Article  CAS  Google Scholar 

  • Cha YJ, Cadwallader KR (1995) Volatile components in salt-fermented fish and shrimp pastes. J Food Sci 60(1):19–24

    Article  CAS  Google Scholar 

  • Eakpetch P, Benjakul S, Visessanguan W, Kijroongrojana K (2008) Autolysis of Pacific white shrimp (Litopenaeousvannamei) meat: characterization and the effect of protein additive. J Food Sci 73(2):95–103

    Article  Google Scholar 

  • Faithong N, Benjakul S, Phatcharat S, Binsan W (2010) Chemical composition and antioxidative activity of Thai traditional fermented shrimp and krill products. Food Chem 119(1):133–140

    Article  CAS  Google Scholar 

  • Garcia-Carreño FL, Hernandez-Cortes MP, Haard NF (1994) Enzyme with peptidase and proteinase activity from the digestive systems of a freshwater and a marine decapod. J Agric Food Chem 42:1456–1461

    Article  Google Scholar 

  • Giri A, Osako K, Okamoto A, Okazaki E, Ohshima T (2011) Antioxidative properties of aqueous and aroma extracts of squid miso prepared with Aspergillus oryzae-inoculated koji. Food Res Int 44:317–325

    Article  CAS  Google Scholar 

  • Hartmann R, Meisel H (2007) Food-derived peptides with biological activity: from research to food applications. Curr Opin Biotechnol 18:163–169

    Article  CAS  Google Scholar 

  • Ichimura T, Hu J, Aita DQ, Maruyama S (2003) Angiotensin I-converting enzyme inhibitory activity and insulin secretion stimulative activity of fermented fish sauce. J Biosci Bioeng 96:496–499

    Article  CAS  Google Scholar 

  • Je JY, Park JY, Jung WK, Park PJ, Kim SK (2005a) Isolation of angiotensin I converting enzyme (ACE) inhibitor from fermented oyster sauce, Crassostrea gigas. Food Chem 90:809–814

    Article  CAS  Google Scholar 

  • Je JY, Park PJ, Byun HG, Jung WK, Kim SK (2005b) Angiotensin I converting enzyme (ACE) inhibitory peptide derived from the sauce of fermented blue mussel, Mytilus edulis. Bioresour Technol 96:1624–1629

    Article  CAS  Google Scholar 

  • Jung WK, Rajapakse N, Kim SK (2004) Antioxidative activity of low molecular weight peptide derived from the sauce of fermented blue mussel, Mytilus edulis. Eur Food Res Technol 220:535–539

    Article  Google Scholar 

  • Kiesvaara M (1975) On the soluble nitrogen fraction of barrel-salted herring and semi-preserves during ripening. Publication No. 10, Technical Research Center of Finland, Helsinki, Finland

  • Kim JS, Shahidi F, Heu MS (2003) Characterization of salt-fermented sauces from shrimp processing by products. J Agric Food Chem 51:784–792

    Article  CAS  Google Scholar 

  • Kobayashi T, Kajiwara M, Wahyuni M, Kitakado T, Hamada-Sato N, Imada C, Watanabe E (2003) Isolation and characterization of halophilic lactic acid bacteria isolated from “terasi” shrimp paste: a traditional fermented seafood products in Indonesia. J Gen Appl Microbiol 49(5):279–286

    Article  CAS  Google Scholar 

  • Kuete V, Tangmouo JG, Penlap Beng V, Ngounou FN, Lontsi D (2006) Antimicrobial activity of the methanolic extract from the stem bark of Tridesmostemon omphalocarpoides (Sapotaceae). J Ethnopharmacol 104:5–11

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  Google Scholar 

  • Li SJ, Seymour TA, Morrissey MT (1994) Isolation of a natural antioxidant from shrimp waste. In: Shahidi F (ed) Natural antioxidants and their uses in foods. American Chemical Society, Washington DC, pp 283–295

    Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193(1):265–275

    CAS  Google Scholar 

  • Oyaizu M (1986) Studies on products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–314

    Article  CAS  Google Scholar 

  • Papagianni M, Anastasiadou S (2009) Pediocins: the bacteriocins of Pediococci. Sources, production, properties and applications. Microb Cell Fac 8(3). doi:10.1186/1475-2859-8-3

  • Pengchumrus W, Upanoi T (2005) Species and distribution of Acetes shrimps in seagrass beds and mangrove canals in the Andaman sea. Technical Paper No. 12/2005, Phuket Marine Biological Center, Department of Marine and Coastal Resources, Ministry of Natural Resources and Environment, Phuket, Thailand

  • Peralta E, Hatate H, Watanabe D, Kawabe D, Murata H, Hama Y, Tanaka R (2005) Antioxidative activity of Philippine salt-fermented shrimp paste and variation of its contents during fermentation. J Oleo Sci 54:553–558

    Article  CAS  Google Scholar 

  • Peralta E, Hatate H, Kawabe D, Kuwahara R, Wakamatsu S, Murata H (2008) Improving antioxidant activity and nutritional components of Philippine salt-fermented shrimp paste through prolonged fermentation. Food Chem 11(1):72–77

    Article  Google Scholar 

  • Phithakpol B (1993) Fish fermentation technology in Thailand. In: Lee CH, Steinkraus KH, Reilly PJA (eds) Fish fermentation technology. United Nations University Press, Seoul, pp 155–166

    Google Scholar 

  • Rajapakse N, Mendis E, Jung WK, Je JY, Kim SK (2005) Purification of a radical scavenging peptide from fermented mussel sauce and its antioxidant properties. Food Res Int 38:175–182

    Article  CAS  Google Scholar 

  • Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med 26(9/10):1231–1237

    Article  CAS  Google Scholar 

  • Sachindra NM, Sato E, Maeda H, Hosokawa M, Niwano Y, Kohno M, Miyashita K (2007) Radical scavenging and singlet oxygen quenching activity of marine carotenoid fucoxanthin and its metabolites. J Agric Food Chem 55:8516–8522

    Article  CAS  Google Scholar 

  • Sarath G, de la Motte RS, Wagner FW (1990) Protease assay methods. In: Beynon RJ (ed) Proteolytic enzymes: a practical approach. IRL Press, Oxford, pp 25–54

    Google Scholar 

  • Seok SH, Park JH, Cho SA, Choi SA, Park JH (2004) Cholesterol lowering effect of SG-GN3, the extract of salted and fermented shrimps, Acetes japonicas, in Triton WR-1339 or high cholesterol-diet induced hypercholesterolemic rats. J Ethnopharmacol 91:231–235

    Article  Google Scholar 

  • Sriket C, Benjakul S, Visessanguan W (2011) Characterisation of proteolytic enzymes from muscle and hepatopancreas of fresh water prawn (Macrobrashium rosenbergii). J Sci Food Agric 91:52–59

    Article  CAS  Google Scholar 

  • Tamang JP, Kailaspathy K (2010) Fermented foods and beverages of the world. CRC Press, Florida

    Book  Google Scholar 

  • Tanasupawat S, Visessanguan W (2008) Thai fermented foods. In: Farnworth ER (ed) Handbook of fermented functional foods, 2nd edn. CRC Press, Florida, pp 495–512

    Google Scholar 

  • Udomsil N, Rodtong S, Tanasupawat S, Yongsawatdigul J (2010) Proteinase-producing halophilic lactic acid bacteria isolated from fish sauce fermentation and their ability to produce volatile compounds. Int J Food Microbiol 141(3):186–194

    Article  CAS  Google Scholar 

  • van Amsterdam FT, Roveri A, Maiorino M, Ratti E, Ursini F (1992) Lacidipine: a dihydropyridine calcium antagonist with antioxidant activity. Free Radic Biol Med 12:183–187

    Article  Google Scholar 

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Acknowledgments

The authors are grateful to the Thailand Research Fund (TRF) and King Mongkut’s University of Technology Thonburi (KMUTT) for a PhD scholarship of Miss Thanyaporn Kleekayai under the Royal Golden Jubilee Ph.D. Program (Grant No. PHD/0133/2552). We thank to the Asian Core Program (ACP), “Capacity Building and Development of Microbial Potential and Fermentation Technology towards New Era”, of Japan Society for the Promotion of Science (JSPS) for the support of research cooperation and the Royal Thai Government for the financial support through research budget of KMUTT. We would finally like to thank Miss Elizabeth Finnegan and Dr. Celia Conessa from University of Limerick, Ireland, for editing and proofing our manuscript.

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The authors declare no conflict of interest.

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Correspondence to Worapot Suntornsuk.

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Kleekayai, T., Saetae, D., Wattanachaiyingyong, O. et al. Characterization and in vitro biological activities of Thai traditional fermented shrimp pastes. J Food Sci Technol 52, 1839–1848 (2015). https://doi.org/10.1007/s13197-014-1528-y

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  • DOI: https://doi.org/10.1007/s13197-014-1528-y

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