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The influence of different polymers on viability of Bifidobacterium lactis 300b during encapsulation, freeze-drying and storage

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Abstract

Seven different types of natural polymers namely hydroxypropyl methylcellulose (HPMC), sodium-carboxymethyl cellulose (Na-CMC), microcrystalline cellulose (MCC), starch BR-07, starch BR-08, dextrin and pullulan were used in order to develop the optimal formula for the entrapment of Bifidobacterium lactis 300B in Ca-alginate based granules. Laminar flow drip casting with Brace-Encapsulator was used in order to prepare the granules. The results showed that alginate/pullulan and alginate/HPMC formulation provide high protection for the bacterial strain used for encapsulation. These two formulations were further used to obtain freeze dried granules, for which the viability in time and at different temperatures was tested. The final results showed a higher viability than the level of the therapeutic minimum (>107 CFU/g) after 15 days of storage. Other parameters like entrapment efficiency, production rate, sphericity, flowability were also discussed.

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Acknowledgments

This work was carried out at Brace GmbH - Karlstein am Main, Germany. The authors would like to thank Dr. Holger Strohm and Mr. Manfred Stöckl for their helpful collaboration during the experimental work.

Declaration of interest

The authors declare that they have no conflicts of interest in concerning this article. This paper was published under the frame of European Social Fund, Human Resources Development Operational Programme 2007-2013, project no. POSDRU/159/1.5/S/132765. The authors are responsible for the content and writing of the article.

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Authors and Affiliations

  1. Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Manastur str., Cluj-Napoca, 400372, Romania

    Oana Lelia Pop, Dan Cristian Vodnar & Carmen Socaciu

  2. Brace GmbH, Am Mittelberg 5, Karlstein am Main, D-63791, Germany

    Thorsten Brandau & Jens Schwinn

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  1. Oana Lelia Pop
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  2. Thorsten Brandau
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  3. Jens Schwinn
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  4. Dan Cristian Vodnar
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  5. Carmen Socaciu
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Correspondence to Oana Lelia Pop or Carmen Socaciu.

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Pop, O.L., Brandau, T., Schwinn, J. et al. The influence of different polymers on viability of Bifidobacterium lactis 300b during encapsulation, freeze-drying and storage. J Food Sci Technol 52, 4146–4155 (2015). https://doi.org/10.1007/s13197-014-1441-4

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  • DOI: https://doi.org/10.1007/s13197-014-1441-4

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