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Effect of combination of dietary fish protein and fish oil on lipid metabolism in rats

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Abstract

This study examined the effects of fish protein in combination with fish oil on rat lipid metabolism. Male Wistar rats were divided into four groups and fed an AIN93G-based diet with casein (20%) + soybean oil (7%), casein (10%) + fish protein (10%) + soybean oil (7%), casein (20%) + soybean oil (5%) + fish oil (2%), and casein (10%) + fish protein (10%) + soybean oil (5%) + fish oil (2%) for 4 weeks. The dietary combination of fish protein and fish oil decreased the contents of serum triacylglycerol, serum cholesterol, liver triacylglycerol and liver cholesterol in addition to altering liver lipid fatty acid composition. These effects are partly due to the increase in fecal cholesterol, bile acid excretion, and increased enzyme activities of fatty acid β-oxidation in the liver. These data suggest that combined intake of fish protein and fish oil lead to both hypocholesterolemic and hypotriglyceridemic in serum and the liver, while sole intake of fish protein or fish oil decrease only cholesterol and triglyceride levels, respectively. These results suggest that combined intake of fish protein and fish oil may play beneficial roles in the prevention of lifestyle-related diseases as compared with sole fish protein intake.

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References

  • Berge RK, Madsen L, Vaagenes H, Tronstad KJ, Göttlicher M, Rustan AC (1999) In contrast with docosahexaenoic acid, eicosapentaenoic acid and hypolipidaemic derivatives decrease hepatic synthesis and secretion of triacylglycerol by decreased diacylglycerol acyltransferase activity and stimulation of fatty acid oxidation. Biochem J 343:191–197

    Article  CAS  Google Scholar 

  • Bligh EG, Dyer WJ (1959) A rapid method of total lipid extraction and purification. Can J Biochem Physiol 37:911–917

    Article  CAS  Google Scholar 

  • Brandsch C, Shukla A, Hirche F, Stangl GI, Eder K (2006) Effect of proteins from beef, pork, and turkey meat on plasma and liver lipids of rats compared with casein and soy protein. Nutrition 22:1162–1170

    Article  CAS  Google Scholar 

  • Bruusgaard A, Sorensen H, Gilhuus-Moe CC, Skålhegg BA (1977) Bile acid determination with different preparations of 3α-hydroxysteroid dehydrogenase. Clin Chim Acta 77:387–395

    Article  CAS  Google Scholar 

  • Cromwell WC, Otvos JD (2004) Low-density lipoprotein particle number and risk for cardiovascular disease. Curr Atheroscler 6:381–387

    Article  Google Scholar 

  • Frøyland L, Vaagenes H, Asiedu DK, Garras A, Lie O, Berge RK (1996) Chronic administration of eicosapentaenoic acid and docosahexaenoic acid as ethyl esters reduced plasma cholesterol and changed the fatty acid composition in rat blood and organs. Lipids 31:169–178

    Article  Google Scholar 

  • Frøyland L, Madsen L, Vaagenes H, Totland GK, Auwerx J, Kryvi H, Staels B, Berge RK (1997) Mitochondrion is the principal target for nutritional and pharmacological control of triglyceride metabolism. J Lipid Res 38:1851–1858

    Google Scholar 

  • Grønn M, Christensen E, Hagve TA, Christophersen BO (1992) Effects of dietary purified eicosapentaenoic acid (20:5 (n-3)) and docosahexaenoic acid (22:6(n-3)) on fatty acid desaturation and oxidation in isolated rat liver cells. Biochim Biophys Acta 1125:35–43

    Article  Google Scholar 

  • Horton JD, Bashmakov Y, Shimomura I, Shimano H (1998) Regulation of sterol regulatory element binding proteins in livers of fasted and refed mice. Proc Natl Acad Sci USA 95:5987–5992

    Article  CAS  Google Scholar 

  • Hosomi R, Fukunaga K, Arai H, Nishiyama T, Yoshida M (2009) Effects of dietary fish protein on serum and liver lipid concentrations in rats and the expression of hepatic genes involved in lipid metabolism. J Agric Food Chem 57:9256–9262

    Article  CAS  Google Scholar 

  • Ide T, Watanabe M, Sugano M, Yamamoto I (1987) Activities of liver mitochondrial and peroxisomal fatty acid oxidation enzymes in rats fed trans fat. Lipids 22:6–10

    Article  CAS  Google Scholar 

  • Ide T, Hong DD, Ranasinghe P, Takahashi Y, Kushiro M, Sugano M (2004) Interaction of dietary fat types and sesamin on hepatic fatty acid oxidation in rats. Biochim Biophys Acta 1682:80–91

    Article  CAS  Google Scholar 

  • Ikeda I, Kudo M, Hamada T, Nagao K, Oshiro Y, Kato M, Sugawara T, Yamahira T, Ito H, Tamaru S, Sato M, Imaizumi K, Nagaoka S, Yanagita T (2009) Dietary soy protein isolate and its undigested high molecular fraction upregulate hepatic ATP-binding cassette transporter G5 and ATP-binding cassette transporter G8 mRNA and increase biliary secretion of cholesterol in rats. J Nutr Sci Vitaminol (Tokyo) 55:252–256

    Article  CAS  Google Scholar 

  • Jacobson TA, Miller M, Schaefer EJ (2007) Hypertriglyceridemia and cardiovascular risk reduction. Clin Ther 29:763–777

    Article  CAS  Google Scholar 

  • Katan MB, Vroomen LH, Hermus RJ (1982) Reduction of casein-induced hypercholesterolemia and atherosclerosis in rabbits and rats by dietary glycine, arginine and alanine. Atherosclerosis 43:381–391

    Article  CAS  Google Scholar 

  • Kelley DS, Kletzien RF (1984) Ethanol modulation of the hormonal and nutritional regulation of glucose 6-phosphate dehydrogenase activity in primary cultures of rat hepatocytes. Biochem J 217:543–549

    CAS  Google Scholar 

  • Kelley DS, Nelson GJ, Hunt JE (1986) Effect of prior nutritional status on the activity of lipogenic enzymes in primary monolayer cultures of rat hepatocytes. Biochem J 235:87–90

    CAS  Google Scholar 

  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    CAS  Google Scholar 

  • Madani S, Lopez S, Blond JP, Prost J, Belleville J (1998) Highly purified soybean protein is not hypocholesterolemic in rats but stimulates cholesterol synthesis and excretion and reduces polyunsaturated fatty acid biosynthesis. J Nutr 128:1084–1091

    CAS  Google Scholar 

  • Markwell MA, McGroarty EJ, Bieber LL, Tolbert NE (1973) The subcellular distribution of carnitine acyltransferases in mammalian liver and kidney. A new peroxisomal enzyme. J Biol Chem 248:3426–3432

    CAS  Google Scholar 

  • Minoura T, Takata T, Sakaguchi M, Takada H, Yamamura M, Hioki K, Yamamoto M (1988) Effect of dietary eicosapentaenoic acid on azoxymethane-induced colon carcinogenesis in rats. Cancer Res 48:4790–4794

    CAS  Google Scholar 

  • Osler M, Helms-Andreasen A, Heitmann B, Høidrup S, Gerdes U, Mørch-Jørgensen L, Schroll M (2002) Food intake patterns and risk of coronary heart disease: a prospective cohort study examining the use of traditional scoring techniques. Eur J Clin Nutr 56:568–574

    Article  CAS  Google Scholar 

  • Pan DA, Hulbert AJ, Storlien LH (1994) Dietary fats, membrane phospholipids and obesity. J Nutr 124:1555–1565

    CAS  Google Scholar 

  • Paton CM, Ntambi JM (2009) Biochemical and physiological function of stearoyl-CoA desaturase. Am J Physiol Endocrinol Metab 297:E28–E37

    Article  CAS  Google Scholar 

  • Pereira MA, O’Reilly E, Augustsson K, Fraser GE, Goldbourt U, Heitmann BL, Hallmans G, Knekt P, Liu S, Pietinen P, Spiegelman D, Stevens J, Virtamo J, Willett WC, Ascherio A (2004) Dietary fiber and risk of coronary heart disease: a pooled analysis of cohort studies. Arch Intern Med 164:370–376

    Article  Google Scholar 

  • Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951

    CAS  Google Scholar 

  • Sabeva N, Liu J, Graf GA (2009) The ABCG5 ABCG8 sterol transporter and phytosterols: implications for cardiometabolic disease. Curr Opin Endocrinol Diab Obes 16:172–177

    Article  CAS  Google Scholar 

  • Serougne C, Ferezou J, Rukaj A (1984) Effect of excess dietary L-cystine on the rat plasma lipoproteins. Ann Nutr Metab 28:311–320

    Article  CAS  Google Scholar 

  • Shukla A, Bettzieche A, Hirche F, Brandsch C, Stangl GI, Eder K (2006) Dietary fish protein alters blood lipid concentrations and hepatic genes involved in cholesterol homeostasis in the rat model. Br J Nutr 96:674–682

    CAS  Google Scholar 

  • Simopoulos AP (2002) Omega-3 fatty acids in inflammation and autoimmune diseases. J Am Coll Nutr 21:495–505

    CAS  Google Scholar 

  • Spielmann J, Noatsch A, Brandsch C, Stangl GI, Eder K (2008) Effects of various dietary arginine and lysine concentrations on plasma and liver cholesterol concentrations in rats. Ann Nutr Metab 53:223–233

    Article  CAS  Google Scholar 

  • Sugano M, Goto S, Yamada Y, Yoshida K, Hashimoto Y, Matsuo T, Kimoto M (1990) Cholesterol-lowering activity of various undigested fractions of soybean protein in rats. J Nutr 120:977–985

    CAS  Google Scholar 

  • Sugiyama K, Ohkawa S, Muramatsu K (1986) Relationship between amino acid composition of diet and plasma cholesterol level in growing rats fed a high cholesterol diet. J Nutr Sci Vitaminol (Tokyo) 32:413–423

    Article  CAS  Google Scholar 

  • Wergedahl H, Liaset B, Gudbrandsen OA, Lied E, Espe M, Muna Z, Mørk S, Berge RK (2004) Fish protein hydrolysate reduces plasma total cholesterol, increases the proportion of HDL cholesterol, and lowers acyl-CoA:cholesterol acyltransferase activity in liver of Zucker rats. J Nutr 134:1320–1327

    CAS  Google Scholar 

  • Yanagita T, Wang YM, Nagao K, Ujino Y, Inoue N (2005) Conjugated linoleic acid-induced fatty liver can be attenuated by combination with docosahexaenoic acid in C57BL/6N mice. J Agric Food Chem 53:9629–9633

    Article  CAS  Google Scholar 

  • Zhang X, Beynen AC (1993) Influence of dietary fish proteins on plasma and liver cholesterol concentrations in rats. Br J Nutr 69:767–777

    Article  CAS  Google Scholar 

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Acknowledgements

We thank Dr. Hayato Maeda of Hirosaki University, for assistance with the Real-time PCR analyses and Kenta Hayashi and Takanori Shimizu of Kansai University for their help with animal care.

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Authors and Affiliations

  1. Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita, Osaka, 564–8680, Japan

    Ryota Hosomi, Kenji Fukunaga & Munehiro Yoshida

  2. Division of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido, 090–8507, Japan

    Hirofumi Arai

  3. Department of Public Health, Kansai Medical University, Moriguchi, Osaka, 570–8506, Japan

    Seiji Kanda & Toshimasa Nishiyama

Authors
  1. Ryota Hosomi
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  2. Kenji Fukunaga
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  3. Hirofumi Arai
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  4. Seiji Kanda
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  5. Toshimasa Nishiyama
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  6. Munehiro Yoshida
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Correspondence to Kenji Fukunaga.

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Hosomi, R., Fukunaga, K., Arai, H. et al. Effect of combination of dietary fish protein and fish oil on lipid metabolism in rats. J Food Sci Technol 50, 266–274 (2013). https://doi.org/10.1007/s13197-011-0343-y

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  • DOI: https://doi.org/10.1007/s13197-011-0343-y

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