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Response of antioxidant defense system in copepod Calanus sinicus Brodsky exposed to CO2-acidified seawater

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Abstract

Marine zooplankton responds sensitively to elevated seawater CO2 concentration. However, the underlying physiological mechanisms have not been studied well. We therefore investigated the effects of elevated CO2 concentration (0.08%, 0.20%, 0.50% and 1.00%) on antioxidant defense components, as well as two detoxification enzymes of Calanus sinicus (copepod). The results showed that glutathione peroxidase (GPx) activity exposed to CO2-acidified seawater was significantly stimulated while other antioxidant components, including glutathione-S-transferase (GST) activity, superoxide dismutase (SOD) activity decreased significantly with reduced glutathione (GSH) level and GSH/oxidized glutathione (GSSG) value. CO2-acidified seawater exhibited stimulatory effects on adenosine triphosphatase (ATPase) activity and acetylcholinesterase (AchE) activity was inhibited. Moreover, the results of principal component analysis indicated that 75.93% of the overall variance was explained by the first two principal components. The elevated CO2 concentration may affect the metabolism and survivals of copepods through impacts these enzymes activities. Further studies are needed to focus on the synergistic effects of elevated CO2 concentration and other environmental factors on copepods.

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Authors and Affiliations

  1. Key Laboratory for Aquatic Ecology and Aquaculture of Tianjin, College of Fisheries, Tianjin Agricultural University, Tianjin, 300384, China

    Dajuan Zhang

  2. College of Ocean and Earth Science, Xiamen University, Xiamen, 361005, China

    Donghui Guo, Guizhong Wang & Shaojing Li

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  1. Dajuan Zhang
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  2. Donghui Guo
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  3. Guizhong Wang
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  4. Shaojing Li
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Correspondence to Shaojing Li.

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Zhang, D., Guo, D., Wang, G. et al. Response of antioxidant defense system in copepod Calanus sinicus Brodsky exposed to CO2-acidified seawater. Acta Oceanol. Sin. 35, 82–88 (2016). https://doi.org/10.1007/s13131-016-0870-5

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  • DOI: https://doi.org/10.1007/s13131-016-0870-5

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