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Biogeographic patterns in the chromosomal distribution of a satellite DNA in the banded tetra Astyanax fasciatus (Teleostei: Characiformes)

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Abstract

The As-51 satellite DNA is a transposon-like sequence formerly described for arthropods and physically identifiable by fluorescent in situ hybridization. In the present work, we describe the occurrence of this sequence, as well the C-banding and karyotype composition, in populations of the group Astyanax fasciatus from Mogi-Guaçu (Araras-SP), Paranapanema (Angatuba and Pilar do Sul-SP), Ribeira de Iguape (Sete Barras-SP) and Tietê (Indaiatuba and Salesópolis-SP) river basins. The specimens from Sete Barras (10 M + 20SM + 12ST + 6A) and Araras (8 M + 22SM + 12ST + 6A) have 2n = 48 chromosomes. The samples from Angatuba, Pilar do Sul and Indaiatuba presented 2n = 46 chromosomes (12 M + 20SM + 10ST + 4A). The individuals collected in Salesópolis showed three cytotypes, bearing 2n = 46 (12 M + 20SM + 10ST + 4A), 2n = 48 (8 M + 22SM + 12ST + 6A) and 2n = 50 (8 M + 16SM + 14ST + 12A). C-banding revealed large heterochromatic blocks at terminal chromosomal regions in all populations and/or cytotypes. All analyzed populations have conspicuous blocks carrying the As-51 satellite DNA, although the number of chromosomes bearing this repetitive sequence was variable among them. Such differences were not related to the diploid number of individuals, but rather to a biogeographic pattern. Aspects of the karyotype evolution and distribution of this sequence in distinct populations are discussed.

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Acknowledgments

The authors wish to thank Malabarba LR and Bertaco V, for the taxonomical identification; Lopes CE for helping with the samples; Smarzaro RS for helping with the GIS software; and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) for the financial support.

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Authors and Affiliations

  1. Laboratório de Genética Ecológica e Evolutiva, Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Campus de Rio Paranaíba. Rodovia BR 354 - km 310, PO Box 22, ZIP 38810-000, Rio Paranaíba, MG, Brazil

    Karine Frehner Kavalco & Rubens Pazza

  2. Université Libre de Bruxelles, Campus de la Plaine, Boulevard du Triomphe, ZIP B-1050, Brussels, Belgium

    Karina de Oliveira Brandão

  3. Universidade de São Paulo. Instituto de Biociências, Rua do Matão, 277 - Edifício André Dreyfus, Cidade Universitária, ZIP 05508090, São Paulo, SP, Brazil

    Lurdes Foresti de Almeida-Toledo

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  1. Karine Frehner Kavalco
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  3. Karina de Oliveira Brandão
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  4. Lurdes Foresti de Almeida-Toledo
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Correspondence to Karine Frehner Kavalco.

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Kavalco, K.F., Pazza, R., de Oliveira Brandão, K. et al. Biogeographic patterns in the chromosomal distribution of a satellite DNA in the banded tetra Astyanax fasciatus (Teleostei: Characiformes). Org Divers Evol 13, 67–76 (2013). https://doi.org/10.1007/s13127-012-0100-8

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