Skip to main content

Advertisement

SpringerLink
Log in

Cardioprotective effect of linseed oil against isoproterenol-induced myocardial infarction in Wistar rats: a biochemical and electrocardiographic study

  • Original Paper
  • Published:
Journal of Physiology and Biochemistry Aims and scope Submit manuscript

Abstract

The present study was designed to evaluate the cardioprotective effect of Tunisian flaxseed oil (Linum usitatissimum) against isoproterenol-induced myocardial infarction in rats by studying hypertensive and cardiac damage markers especially electrocardiographic changes and troponin T serum level. In vitro, the extracted oil showed an important inhibition of angiotensin converting enzyme (ACE) with an IC50 = 85.96 μg/ml. According to chemical analysis, this extract is composed essentially of alpha linolenic acid (ALA), an n-3 polyunsaturated fatty acid (58.59 %). Male rats were randomly divided into three groups, namely control (C), isoproterenol (ISO), and isoproterenol-treated group with flaxseed oil (FO + ISO). Isoproterenol injection showed changes in ECG pattern, including ST-segment elevation (diagnostic of myocardial infarction), increase in the serum levels of Troponin T and cardiac injury markers (creatine kinase-MB (CK-MB), lactate dehydrogenase (LDH), alkaline phosphatase (ALP), aspartate transaminase (AST), and alanine transaminase (ALT)). However, Linum oil pre-co-treatment prevented almost all the parameters isoproterenol-induced myocardial infarction in rats. Results of the present study proved that flaxseed oil has a significant effect by heart protection against isoproterenol-induced myocardial infarction through beneficial effect of the important fraction of ALA.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Balazs T, Ferrans VJ (1978) Cardiac lesions induced by chemicals. Environ Health Perspect 26:181–191

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  2. Baroldi G (1974) Myocardial necrosis: the need for definition. J Mol Cell Cardiol 6:401–402

    Article  CAS  PubMed  Google Scholar 

  3. Chen JP, Stavro M, Thompson LU (2002) Dietary flaxseed inhibits human breast cancer growth and metastasis and down regulates expression of insulin-like growth factor and epidermal growth factor receptor. Nutr Cancer 43:187–192

    Article  CAS  PubMed  Google Scholar 

  4. Collins TF, Sprando RL, Black TN, Olejnik N, Wiesenfeld PW, Babu US, Bryant M, Flynn TJ, Ruggles DI (2003) Effects of flaxseed and defatted flaxseed meal on reproduction and development in rats. Food Chem Toxicol 41:819–834

    Article  CAS  PubMed  Google Scholar 

  5. Finegold JA, Asaria P, Francis DP (2013) Mortality from ischaemic heart disease by country, region, and age: Statistics from World Health Organisation and United Nations. Int J Cardiol 168:934–945

    Article  PubMed Central  PubMed  Google Scholar 

  6. Ganapathy P, Rajadurai M, Ashokumar N (2014) Cardioprotective effect of β-sitosterol on lipid peroxides and antioxidant in isoproterenol-induced myocardial infarction in rats: a histopathological study. Int J Curr Res 6:7260–7266

    CAS  Google Scholar 

  7. Grimm D, Elsner D, Schunkert H, Pfeifer M, Griese D, Bruckschlegel G, Muders F, Riegger GA, Kromer EP (1998) Development of heart failure following isoproterenol administration in the rat: role of renin-angiotensin system. Cardiovasc Res 37:91–100

    Article  CAS  PubMed  Google Scholar 

  8. Herchi W, Harrabi S, Sebei K, Rochut S, Boukhchina S, Pepe C, Kallel H (2009) Phytosterols accumulation in the seeds of Linum usitatissimum L. Plant Physiol Biochem 47:880–885

    Article  CAS  PubMed  Google Scholar 

  9. Jaffe AS, Ravkilde J, Roberts R, Naslund U, Apple FS, Galvani M, Katus H (2000) It’s time for a change to a troponin standard. Circulation 102:1216–1220

    Article  CAS  PubMed  Google Scholar 

  10. Karthick M, Prince P (2006) Preventive effect of rutin, a bioflavonoid, on lipid peroxides and antioxidants in ISO-induced myocardial infarction in rats. J Pharm Pharmacol 58:701–707

    Article  CAS  PubMed  Google Scholar 

  11. Kinniry P, Amrani Y, Vachani A, Solomides CC, Arguiri E, Workman A, Carter J, Christofidou-Solomidou M (2006) Dietary flaxseed supplementation ameliorates inflammation and oxidative tissue damage in experimental models of acute lung injury in mice. J Nutr 136:1545–1551

    CAS  PubMed  Google Scholar 

  12. Lozano R, Naghavi M, Foreman K, Lim S, Shibuya K, Aboyans V, Cross M (2012) Global and regional mortality from 235 causes of death for 20 age groups in 1990 and 2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet 380:2095–2128

    Article  PubMed  Google Scholar 

  13. Marambe KH, Shand PJ, Wanasundara JP (2011) Release of angiotensin I-converting enzyme inhibitory peptides from flaxseed (Linum usitatissimum L.) protein under simulated gastrointestinal digestion. J Agric Food Chem 59:9596–604

    Article  CAS  PubMed  Google Scholar 

  14. Meier MA, Al-Badr WH, Cooper JV, Klin-Rogers EM, Smith DE, Eagle KA et al (2002) The new definition of myocardial infarction: diagnostic and prognostic implications in patients with acute coronary syndromes. Arch Intern Med 162:1585–1589

    Article  PubMed  Google Scholar 

  15. Milei J, Nunez RG, Rapaport M (1978) Pathogenesis of isoproterenol induced myocardial lesions its relation to human coagulate myocytolysis. Cardiology 63:139–151

    Article  CAS  PubMed  Google Scholar 

  16. Morrow DA, Cannon CP, Jesse RL, Newby LK, Ravnkilde J, Storrow AB, Wu AH, Tang W (2007) National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: clinical characteristics and utilization of biochemical markers of acute coronary syndromes. Clin Chem 53:552–574

    Article  CAS  PubMed  Google Scholar 

  17. Mozaffarian D (2005) Does alpha-linolenic acid intake reduce the risk of coronary heart disease? A review of the evidence. Altern Ther Health Med 11:24–30

    PubMed  Google Scholar 

  18. Nora JJ, Lortscher RH, Spangler RD, Nora AH, Kimberling WJ (1980) Genetic-epidemiologic study of early-onset ischemic heart disease. Circulation 61:503–508

    Article  CAS  PubMed  Google Scholar 

  19. Panda VS, Naik SR (2008) Cardioprotective activity of Ginkgo biloba phytosomes in isoproterenol induced myocardial necrosis in rats: a biochemical and histoarchitectural evaluation. Exp Toxicol Pathol 60:397–404

    Article  PubMed  Google Scholar 

  20. Patel DK, Desai SN, Gandhi HP, Devkar RV, Ramachandran AV (2012) Cardioprotective effect of Coriandrum sativum L. on isoproterenol induced myocardial necrosis in rats. Food Chem Toxicol 50:3120–3125

    Article  CAS  PubMed  Google Scholar 

  21. Plat J, Mensink RP (2005) Plant stanol and sterol esters in the control of blood cholesterol levels: mechanism and safety aspects. Am J Cardiol 96:15–22

    Article  Google Scholar 

  22. Prasad K (1997) Dietary flax seed in prevention of hypercholesterolemia atherosclerosis. Atherosclerosis 132:69–76

    Article  CAS  PubMed  Google Scholar 

  23. Prasad K (2000) Oxidative stress as a mechanism of diabetes in diabetic BB prone rats: effect of secoisolariciresinol diglucoside (SDG). Mol Cell Biochem 209:89–96

    Article  CAS  PubMed  Google Scholar 

  24. Quinones M, Miguel M, Aleixandre A (2013) Beneficial effects of polyphenols on cardiovascular disease. Pharmacol Res 68:125–131

    Article  CAS  PubMed  Google Scholar 

  25. Rajadurai M, Mainzen Prince PS (2007) Preventive effect of naringin on isoproterenol-induced cardiotoxicity in Wistar rats: an in vivo and in vitro study. Toxicology 232:216–225

    Article  CAS  PubMed  Google Scholar 

  26. Rajadurai M, Stanely Mainzen Prince P (2007) Preventive effect of naringin on cardiac markers, electrocardiographic patterns and lysosomal hydrolases in normal and isoproterenol-induced myocardial infarction in Wistar rats. Toxicology 230:178–188

    Article  CAS  PubMed  Google Scholar 

  27. Rajesh KN, Murthy C, Kumar KM, Madhusudhan B, Ravishankar GA (2006) Antioxidant potentials of flaxseed by in vivo model. J Agric Food Chem 54:3794–3799

    Article  Google Scholar 

  28. Roger VL, Killian JM, Weston SA, Jaffe AS, Kors J, Santrach PJ, Jacobsen SJ (2006) Redefinition of myocardial infarction: prospective evaluation in the community. Circulation 114:790–797

    Article  PubMed  Google Scholar 

  29. Rona G (1985) Catecholamine cardiotoxicity. J Mol Cell Cardiol 17:291–306

    Article  CAS  PubMed  Google Scholar 

  30. Simopoulos AP (2002) The importance of the ratio of omega-6/omega-3 essential fatty acids. Biomed Pharmacother 56:365–379

    Article  CAS  PubMed  Google Scholar 

  31. Simopoulos AP (2008) The importance of the omega-6/omega-3 fatty acid ratio in cardiovascular disease and other chronic diseases. Exp Biol Med 233:674–688

    Article  CAS  Google Scholar 

  32. Singal PK, Beamish RE, Dhalla NS (1983) Potential oxidative pathways of catecholamines in the formation of lipid peroxides and genesis of heart disease. Adv Exp Med Biol 161:391–401

    Article  CAS  PubMed  Google Scholar 

  33. Wu JP, Aluko RE, Muir AD (2002) Improved method for direct high performance liquid chromatography assay of angiotensin-converting enzyme-catalyzed reactions. J Chromatogr 950:125–30

    Article  CAS  Google Scholar 

  34. Zanwar AA, Hegde MV, Bodhankar SL (2011) Cardioprotective activity of flax lignan concentrate extracted from seeds of Linum usitatissimum in isoprenalin induced myocardial necrosis in rats. Interdisc Toxicol 4(2):90–97

    Article  Google Scholar 

  35. Zhou R, Xu Q, Zheng P, Yan L, Zheng J, Dai G (2008) Cardioprotective effect of fluvastatin on isoproterenol-induced myocardial infarction in rat. Eur J Pharmacol 586:244–250

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

This research was supported by the Tunisian Ministry of Higher Education and Scientific Research and the Tunisian Ministry of Public Health. Authors wish to thank Mbarek Nasri and Chedly Tmar for their assistance and cooperation.

Conflict of interests

The authors declare no conflict of interest.

Author information

Authors and Affiliations

  1. Laboratory of Animal Ecophysiology, Faculty of Sciences of Sfax, University of Sfax, P.O. Box 95, 3052, Sfax, Tunisia

    Amal Derbali, Kais Mnafgui & Abdelfattah El Feki

  2. Laboratory of Chemistry of Natural Products, Faculty of Sciences of Sfax, B.P. 1171, 3000, Sfax, Tunisia

    Marwa Affes & Noureddine Allouche

  3. Internal Medicine Department, Hospital of Sidi Bouzid, Sidi Bouzid, 9100, Tunisia

    Fatma Derbali & Raouf Hajji

  4. Laboratory of Plant Biotechnology, Faculty of Sciences of Sfax, B.P. 1171, 3000, Sfax, Tunisia

    Neji Gharsallah

Authors
  1. Amal Derbali
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kais Mnafgui
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Marwa Affes
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fatma Derbali
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Raouf Hajji
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Neji Gharsallah
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Noureddine Allouche
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Abdelfattah El Feki
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Kais Mnafgui.

Additional information

Amal Derbali and Kais Mnafgui contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Derbali, A., Mnafgui, K., Affes, M. et al. Cardioprotective effect of linseed oil against isoproterenol-induced myocardial infarction in Wistar rats: a biochemical and electrocardiographic study. J Physiol Biochem 71, 281–288 (2015). https://doi.org/10.1007/s13105-015-0411-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13105-015-0411-2

Keywords

Search

Navigation