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Translational Intracerebral Hemorrhage: a Need for Transparent Descriptions of Fresh Tissue Sampling and Preclinical Model Quality

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Abstract

For years, strategies have been proposed to improve translational success in stroke research by improving the quality of animal studies. However, articles that report preclinical intracerebral hemorrhage (ICH) studies continue to lack adequate qualitative and quantitative descriptions of fresh brain tissue collection. They also tend to lack transparency about animal model quality. We conducted a systematic review of 82 ICH research articles to determine the level of detail reported for brain tissue collection. We found that only 24 (29 %) reported the volume, weight, or thickness of tissue collected and a specific description of the anatomical location. Thus, up to 71 % of preclinical ICH research articles did not properly define how fresh specimens were collected for biochemical measurements. Such omissions may impede reproducibility of results between laboratories. Although existing criteria have improved the quality of preclinical stroke studies, ICH researchers need to identify specific guidelines and strategies to avoid pitfalls, minimize bias, and increase reproducibility in this field.

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Acknowledgments

This work was supported by grants from the American Heart Association (13GRNT15730001) and the National Institutes of Health (K01AG031926, R01AT007317, and R01NS078026). We thank Claire Levine, MS, ELS, for assistance with manuscript preparation.

Authors’ Contributions

C-F.C. and J.W. conceived and wrote the paper; C-F.C. and L.C. designed the search strategy and selection criteria; C-F.C., L.C., and J.W. performed article identification and evaluation; and C-F.C. performed hemorrhage models for the representative images. All authors revised the manuscript and read and approved the final version before submission.

Compliance with Ethics Requirements

All institutional and national guidelines for the care and use of laboratory animals were followed.

Conflict of Interest

C-F.C. declares that he has no conflict of interest; C.C. declares that she has no conflict of interest; J. W. declares that he has no conflict of interest.

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Authors and Affiliations

  1. Department of Anesthesiology and Critical Care Medicine, Johns Hopkins University School of Medicine, 720 Rutland Ave, Ross Bldg 370B, Baltimore, MD, 21205, USA

    Che-Feng Chang & Jian Wang

  2. Department of Maternal and Child Health, School of Public Health, Sun Yat-Sen University, Guangzhou, Guangdong, China

    Li Cai

  3. Department of International Health, Johns Hopkins University Bloomberg School of Public Health, Baltimore, MD, 21205, USA

    Li Cai

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  1. Che-Feng Chang
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Correspondence to Jian Wang.

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Chang, CF., Cai, L. & Wang, J. Translational Intracerebral Hemorrhage: a Need for Transparent Descriptions of Fresh Tissue Sampling and Preclinical Model Quality. Transl. Stroke Res. 6, 384–389 (2015). https://doi.org/10.1007/s12975-015-0399-5

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