Abstract
The effect of functional rice giant embryo and Aranghyangchal on the glucose metabolism and antioxidative defense status in high fat-fed C57BL/6N mice were investigated. The animals were randomly divided into four dietary groups: normal control (NC), high fat (HF), and high fat supplemented with giant embryo rice (HF-GE) or Aranghyangchal rice (HF-AR). After 8 weeks of feeding them with experimental diets, the HF mice exhibited a marked increase in the blood glucose concentration, plasma insulin level, and lipid peroxidation and a significant decrease in the hepatic glycogen level relative to the NC group. On the other hand, diet supplementation of the rice samples suppressed this high fat diet-induced hyperglycemia and oxidative stress through inhibition of the glucose-regulating enzymes and activation of the antioxidant enzymes. These findings demonstrate that the giant embryo and Aranghyangchal rice may be beneficial as biomaterials in the development of functional food with hypoglycemic and antioxidative properties.
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Aebi H. Catalase. 1974. Method of Enzymatic Analysis, HU Bergmeyer, ed, Volume 2. Academic Press, New York, pp 673–684
Alegre M, Ciudad CJ, Fillat C, Guinovart JJ. 1988. Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction. Anal. Biochem. 173:185–189
American Institute of Nutrition. 1977. Report of ad hoc committee on standards for nutritional studies. J. Nutr. 107:1340–1348
AOAC. 2003. AOAC Official Methods of Analysis. Virginia: Association of official analytical chemists, Inc.
Bentle LA, Lardy HA. 1976. Interaction of anions and divalent metal ions with phosphoenolpyruvate carboxykinase. J. Biol. Chem. 251:2916–2921
Beyer P. 2010. Golden rice and ‘golden’ crops for human nutrition. New Biotechnol. 27:478–481
Bradford MM. 1976. A rapid sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248–254
Davidson AL, Arion WJ. 1987. Factors underlying significant underestimations of glucokinase activity in crude liver extracts: physiological implications of higher cellular activity. Arch. Biochem. Biophys. 253:156–167
Hulcher FH, Oleson WH. 1973. Simplified spectrophotometric assay for microsomal 3-hydroxy-3-methylglutaryl CoA reductase by measurement of coenzyme A. J. Lipid Res. 14:625–631
Inzucchi SE. 2002. Oral antihyperglycemic therapy for type 2 diabetes: scientific review. J. Am. Med. Assoc. 287:360–372
Lee YR, Kang MY, Nam SH. 2005. Effect of giant embryonic rice supplementation on the lipid peroxidation levels and antioxidative enzyme activities in the plasma and liver of streptozotocin-induced diabetic rats. J. Kor. Soc. Appl. Biol. Chem. 48:358–363
Lee YR, Kim CE, Kang MY, Nam SH. 2007. Cholesterollowering and antioxidant status-improving efficacy of germinated giant embryonic rice (Oryza sativa L.) in high cholesterol-fed rats. Ann. Nutr. Metab. 51:519–526
Lichtenstein AH, Schwab US. 2000. Relationship of dietary fat to glucose metabolism. Atherosclerosis 150:227–243
Mackness MI, Arrol S, Durrington PN. 1991. Paraoxonase prevents accumulation of lipoperoxides in low-density lipoprotein. FEBS Lett. 286:152–154
Maritim AC, Sanders RA, Watkins JB. 2003. Diabetes, oxidative stress, and antioxidants: a review. J. Biochem. Mol. Toxicol. 17:24–38
ai]Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and convenient assay for superoxide dismutase. Eur. J. Biochem. 47:469–474
McCord JM, Fridovich I. 1969. Superoxide dismutase: an enzymic function for erythrocuprein (hemocuprein). J. Biol. Chem. 244:6049–6055
Min B, Gu L, McClung AM, Bergman CJ, Chen MH. 2012. Free and bound total phenolic concentrations, antioxidant capacities, and profiles of proanthocyanidins and anthocyanins in whole grain rice (Oryza sativa L.) of different bran colours. Food Chem. 133:715–722
Mize CE, Langdon RG. 1952. Hepatic glutathione reductase, purification and general kinetic properties. J. Biol. Chem. 237:1589–1595
Mullineaux PM, Creissen GP. 1997. Glutathione reductase: regulation and role in oxidative stress. In JG Scandalios, ed, Oxidative Stress and the Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, New York, pp 667–713
Ng CJ, Shih DM, Hama SY, Villa N, Navab M, Reddy ST. 2005. The paraoxonase gene family and atherosclerosis. Free Radic. Biol. Med. 38:153–163
Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95:351–358
Paglia ED, Valentine WN. 1967. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab Clin. Med. 70:158–169
Reiter RJ, Tan D, Burkhardt S. 2002. Reactive oxygen and nitrogen species and cellular and organismal decline:amelioration with melatonin. Mech. Aging Dev. 123:1007–1019
Seifter S, Dayton S, Navic B, Muntwyler E. 1950. The estimation of glycogen with the anthrone reagent. Arch. Biochem. 25:191–200
Seo WD, Kim JY, Park DS, Han SI, Jang KC et al. 2011. Comparative analysis of physiochemicals and antioxidative properties of new giant embryo mutant, YR23517Acp79, in rice (Oryza sativa L.). J. Kor. Soc. Appl. Biol. Chem. 54:700–709
She P, Shiota M, Shelton KD, Chalkley R, Postic C, Magnuson MA. 2000. Phosphoenolpyruvate carboxykinase is necessary for the integration of hepatic energy metabolism. Mol. Cell Biol. 20:6508–6517
Van Schaftingen E, Gerin I. 2002. The glucose-6-phosphatase system. Biochem. J. 362:513–532
West IC. 2000. Radicals and oxidative stress in diabetes. Diabet. Med. 17:171–180
Zhu L, Gu M, Meng X, Cheung SC, Yu H, Huang J, Sun Y, Shi Y, Liu Q. 2012. High-amylose rice improves indices of animal health in normal and diabetic rats. Plant Biotechnol. J. 10:353–362
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Kang, M.Y., Chung, S.I., Xu, M. et al. Functional rice giant embryo and Aranghyangchal reduce blood glucose level and enhance antioxidative defense status in high fat-fed mice. J. Crop Sci. Biotechnol. 17, 141–146 (2014). https://doi.org/10.1007/s12892-014-0068-3
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DOI: https://doi.org/10.1007/s12892-014-0068-3