Abstract
As species ranges shift due to anthropogenic change, accurate detection of hybridization between species will become increasingly important for conservation biologists. The black-capped (Poecile atricapillus) and Carolina (Poecile carolinensis) chickadee hybrid zone is difficult to study because the parental species possess similar morphologies and song is an unreliable species identifier. Further, the hybrid zone is moving northward rapidly due to environmental change. Here, we present 10 single nucleotide polymorphism markers developed from black-capped and Carolina chickadee transcriptome sequences. This marker set coupled with species-specific restriction enzyme digestion allows fast, easy genotyping of pure species and hybrid individuals within the hybrid zone.
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Acknowledgements
This research was funded by Lehigh University and the Fuller Evolutionary Biology Program at the Cornell Lab of Ornithology. We thank Zac Cheviron and Jennifer Jones for RNA extraction and sequencing. We thank Sean Nelson for assistance in the lab, Irby Lovette for helpful discussion, and the American Museum of Natural History and the Smithsonian National Museum of Natural History for samples.
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McQuillan and Huynh have contributed equally to this work.
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McQuillan, M.A., Huynh, A.V., Taylor, S.A. et al. Development of 10 novel SNP-RFLP markers for quick genotyping within the black-capped (Poecile atricapillus) and Carolina (P. carolinensis) chickadee hybrid zone. Conservation Genet Resour 9, 261–264 (2017). https://doi.org/10.1007/s12686-016-0667-z
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DOI: https://doi.org/10.1007/s12686-016-0667-z