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Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides

  • Microsatellite Letters
  • Published:
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Abstract

The hermaphroditic flat-spired three-tooth land snail (Triodopsis platysayoides) is endemic to a 21-km stretch of the Cheat River Gorge of northeastern West Virginia, USA. We document isolation and characterization of ten microsatellite DNA markers in this at-risk species. The markers displayed a moderate level of allelic diversity (averaging 7.1 alleles/locus) and heterozygosity (averaging 58.6 %). Allelic diversity at seven loci was sufficient to produce unique multilocus genotypes; no indication of selfing was detected in this cosexual species. Minimal deviations from Hardy–Weinberg equilibrium and no linkage disequilibrium were observed within subpopulations. All loci deviated from Hardy–Weinberg expectations when individuals from subpopulations were pooled. Microsatellite markers developed for T. platysayoides yielded sufficient genetic diversity to (1) distinguish all individuals sampled and the level of selfing; (2) be appropriate for addressing fine-scale population structuring; (3) provide novel demographic insights for the species; and (4) cross-amplify and detect allelic diversity in the congeneric T. juxtidens.

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References

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Acknowledgments

This research was funded by the U.S. Geological Survey’s Leetown Science Center (USGS-LSC), the USGS and U.S. Fish and Wildlife Service’s Science Support Program, and the West Virginia Division of Natural Resources, Wildlife Resources Section. Use of trade, product, or firm names does not imply endorsement by the U.S. Government.

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Authors and Affiliations

  1. Aquatic Ecology Branch, Leetown Science Center, U. S. Geological Survey, 11649 Leetown Road, Kearneysville, WV, 25430, USA

    T. L. King & M. S. Eackles

  2. University of North Carolina Wilmington, 601 S. College Rd., Wilmington, NC, 28403, USA

    B. A. Garner, M. van Tuinen & B. S. Arbogast

Authors
  1. T. L. King
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  2. M. S. Eackles
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  3. B. A. Garner
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  4. M. van Tuinen
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  5. B. S. Arbogast
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Corresponding author

Correspondence to T. L. King.

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King, T.L., Eackles, M.S., Garner, B.A. et al. Isolation and characterization of microsatellite DNA loci in the threatened flat-spired three-toothed land snail Triodopsis platysayoides . Conservation Genet Resour 7, 767–769 (2015). https://doi.org/10.1007/s12686-015-0456-0

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  • DOI: https://doi.org/10.1007/s12686-015-0456-0

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