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Development of ten polymorphic microsatellite markers for Scabiosa canescens Waldst. & Kit. (Dipsacaceae)

  • Microsatellite Letters
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Abstract

We developed ten polymorphic microsatellite markers for the herbaceous species Scabiosa canescens Waldst. & Kit. The species is currently not assessed under the IUCN Red List but has been declining historically due to intensification in agriculture and is recently declining due to habitat loss caused by agricultural abandonment of steppe-like dry grasslands. The microsatellite loci were tested for polymorphism on DNA templates extracted from 26 specimens collected across the species’ range in Germany. Across the ten loci, two to eight alleles were detected with an observed and expected heterozygosity ranging from 0.192 to 0.769 and 0.265 to 0.768, respectively. These newly developed markers provide a valuable tool for population genetic investigations required to design effective conservation measures.

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Acknowledgments

The work was financed by the Federal Agency for Nature Conservation (BfN) Germany under the grant number FKZ 3512-86-0400. Illumina sequencing was conducted at the Berlin Center for Genomics in Biodiversity Research (BeGenDiv). This is Publication No. 15 of the BeGenDiv.

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Author notes

  1. Sascha A. Ismail

    Present address: Institute of Biological and Environmental Sciences, University of Aberdeen, Cruickshank Building, St. Machar Drive, Aberdeen, AB24 3UU, Scotland, UK

Authors and Affiliations

  1. Botanischer Garten und Botanisches Museum Berlin-Dahlem, Dahlem Centre of Plant Sciences, Freie Universität Berlin, Königin Luise-Straße 6-8, 14195, Berlin, Germany

    Sascha A. Ismail, Virginia K. Duwe, Helga Fleischer-Notter & Thomas Borsch

Authors
  1. Sascha A. Ismail
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  2. Virginia K. Duwe
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  3. Helga Fleischer-Notter
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  4. Thomas Borsch
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Corresponding author

Correspondence to Sascha A. Ismail.

Appendix

Appendix

See Table 2.

Table 2 Locations and voucher information of sampled populations
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Ismail, S.A., Duwe, V.K., Fleischer-Notter, H. et al. Development of ten polymorphic microsatellite markers for Scabiosa canescens Waldst. & Kit. (Dipsacaceae). Conservation Genet Resour 7, 725–728 (2015). https://doi.org/10.1007/s12686-015-0446-2

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  • DOI: https://doi.org/10.1007/s12686-015-0446-2

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