Skip to main content

Advertisement

SpringerLink
Log in

Isolation and characterization of polymorphic microsatellite markers from a globally distributed marine apex predator, the tiger shark (Galeocerdo cuvier)

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

High levels of fishing have resulted in declines among many of the oceans top predators, including the globally distributed tiger shark (Galeocerdo cuvier). Overexploitation of this species has led to declines in parts of its distribution, which may have important ecological consequences given the tiger shark’s trophic position as a large bodied, generalist predator. To assess the population genetics of this species, an enrichment protocol was used to isolate a suite of nine di- and tetra-nucleotide microsatellite loci within the tiger shark, which were subsequently characterized using genomic DNA derived from 40 individuals sampled from Hawaiian waters. The number of alleles per locus ranged from 3 to 23, and the average expected heterozygosities across loci ranged from 0.30 to 0.93. Analyses suggested a low frequency of null alleles across markers, and all loci conformed to both Hardy–Weinberg and linkage equilibrium at P > 0.01.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Chapuis M-P, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol 24:621–631

    Article  CAS  PubMed  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222

    Article  CAS  PubMed  Google Scholar 

  • Rousset F (2008) Genepop ‘007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365–386

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

  • Simpfendorfer C (2009) Galeocerdo cuvier. IUCN red list of threatened species. http://www.iucnredlist.org/details/39378/0. Accessed 9 Nov 2014

Download references

Acknowledgments

Funding provided by grants from the Save Our Seas Foundation and Guy Harvey Ocean Foundation (MSS), Pritzker Foundation to the Pritzker Laboratory for Molecular Systematics and Evolution (KAF), and a Natural Sciences and Engineering Research Council of Canada (NSERC) Postgraduate Scholarship (AMB). We thank T. Daly-Engel, K. Duncan, D. Grubbs, and B. Wetherbee for samples.

Author information

Authors and Affiliations

  1. Save Our Seas Shark Research Center and Guy Harvey Research Institute, Nova Southeastern University, Oceanographic Center, 8000 North Ocean Drive, Dania Beach, FL, 33004, USA

    A. M. Bernard & M. S. Shivji

  2. Pritzker Laboratory for Molecular Systematics and Evolution, The Field Museum of Natural History, 1400 South Lake Shore Drive, Chicago, IL, 60605, USA

    K. A. Feldheim

Authors
  1. A. M. Bernard
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. K. A. Feldheim
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M. S. Shivji
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. S. Shivji.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 34 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Bernard, A.M., Feldheim, K.A. & Shivji, M.S. Isolation and characterization of polymorphic microsatellite markers from a globally distributed marine apex predator, the tiger shark (Galeocerdo cuvier). Conservation Genet Resour 7, 509–511 (2015). https://doi.org/10.1007/s12686-014-0408-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0408-0

Keywords

Search

Navigation