Skip to main content
SpringerLink
Log in

Development of nuclear and mitochondrial microsatellite markers for the relictual conifer genus Athrotaxis (Cupressaceae)

  • Microsatellite Letters
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

Athrotaxis D. Don (Cupressaceae) is a relictual conifer genus and is comprised of two or three ecologically divergent species endemic to Tasmania. Utilizing RNA-seq data obtained by next generation sequencing techniques, 16 polymorphic EST-SSR (simple sequence repeats in expressed sequence tags) markers with 2–15 alleles in nuclear and mitochondria genomes were developed and characterized. These markers will be used to investigate the speciation and hybridisation processes in the genus, the species’ range-wide genetic structure and diversity and the importance of clonality in maintaining current populations.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Faircloth BC (2008) MSATCOMMANDER: detection of microsatellite repeat arrays and automated, locus-specific primer design. Mol Ecol Resour 8:92–94

    Article  CAS  PubMed  Google Scholar 

  • Goudet J (1995) FSTAT (version 1.2): a computer program to calculate F-statistics. J Hered 86:485–486

    Google Scholar 

  • Hill KD (1998) ‘Gymnosperms’—the paraphyletic stem of seed plants. Flora of Australia. CSIRO, Melbourne, pp 505–526

    Google Scholar 

  • Jordan GJ, Brodribb TJ, Loney PE (2004) Water loss physiology and the evolution within the Tasmanian conifer genus Athrotaxis (Cupressaceae). Aust J Bot 52:765–771

    Article  Google Scholar 

  • Neale DB, Marshall KA, Sederoff RR (1989) Chloroplast and mitochondrial DNA are paternally inherited in Sequoia sempervirens D. Don Endl. Proc Natl Acad Sci USA 86:9347–9349

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Samuel Martins and Stuart MacDonald for assistance in the field, Madoka Hirano for help with processing samples and the Department of Primary Industries, Parks, Water and Environment, Tasmanian Government, for providing collection permits. Funding was provided by the Australian Research Council (DP120100501), a Japan Society for the Promotion of Science Grant-in-Aid for Scientific Research (JSPS KAKENHI 26850098), and the Environment Research and Technology Development Fund of the Ministry of the Environment (4-1403).

Author information

Authors and Affiliations

  1. Laboratory of Plant Evolution and Biodiversity, Department of General Systems Studies, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan

    Shota Sakaguchi & Motomi Ito

  2. School of Biological Sciences, University of Tasmania, Private Bag 55, Hobart, TAS, 7001, Australia

    James R. P. Worth & Gregory J. Jordan

  3. Royal Botanic Garden Edinburgh, 20A Inverleith Row, Edinburgh, EH3 5LR, UK

    Philip Thomas

Authors
  1. Shota Sakaguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. James R. P. Worth
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Gregory J. Jordan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Philip Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Motomi Ito
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shota Sakaguchi.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOC 55 kb)

Appendix

Appendix

Information of the plant samples used for the initial PCR amplification trials

Species

Locality

GPS coordinates

Voucher no.

A. cupressoides D. Don

Mt. Kate, Tasmania

41°38′10.0″S, 145°57′02.8″E

KYO 00019887

A. cupressoides D. Don

Lake Dobson, Tasmania

42°41′05.5″S, 146°35′34.6″E

KYO 00019888

A. cupressoides D. Don

Ouse River, Tasmania

41°53′49.0″S, 146°36′59.7″E

KYO 00019889

A. laxifolia Hook.

Tarn Shelf, Tasmania

42°40′07.5″S, 146°33′37.0″E

KYO 00019890

A. laxifolia Hook.

Tarn Shelf, Tasmania

42°39′27.8″S, 146°34′02.8″E

KYO 00019891

A. selaginoides D.Don

Mt. Black, Tasmania

41°47′04.6″S, 145°35′37.4″E

KYO 00019884

A. selaginoides D. Don

Collingwood River, Tasmania

42°07′27.0″S, 145°49′56.5″E

KYO 00019885

A. selaginoides D. Don

Tarn Shelf, Tasmania

42°40′40.0″S, 146°34′11.0″E

KYO 00019886

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sakaguchi, S., Worth, J.R.P., Jordan, G.J. et al. Development of nuclear and mitochondrial microsatellite markers for the relictual conifer genus Athrotaxis (Cupressaceae). Conservation Genet Resour 7, 477–481 (2015). https://doi.org/10.1007/s12686-014-0400-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-014-0400-8

Keywords

Search

Navigation