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Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos

  • Microsatellite Letters
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Abstract

The milkfish, Chanos chanos, is an important aquaculture resource in Southeast Asia. Using NGS data, 72 microsatellite markers were developed. PCR product confirmation using agarose gel electrophoresis allowed the identification of 24 utilizable markers. Milkfish samples (n = 48) from a hatchery stock in Palawan, Philippines were analyzed for genetic variability at the aforementioned 24 loci. Consequently, nine of these microsatellite loci were noted to have high success rate in amplification, exhibited polymorphism with 19 maximum number of alleles and no null alleles. These are the first microsatellite markers to be developed and characterized for C. chanos that will efficiently enable genetic stock delineation and monitoring as well as marker-aided genetic improvement research.

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Acknowledgments

Funds for this study were provided by the Department of Science and Technology-Philippine Council for Agriculture, Aquatic and Natural Resources Research and Development (DOST-PCAARRD) and the Southeast Asian Fisheries Development Center, Aquaculture Department (SEAFDEC/AQD; study code Br-02-F2012B).

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Authors and Affiliations

  1. Institute of Biology, University of the Philippines Diliman, 1101, Quezon City, Philippines

    Brian S. Santos & Zubaida U. Basiao

  2. Aquaculture Department, Southeast Asian Fisheries Development Center, 1940, Binangonan, Rizal, Philippines

    Maria Rowena R. Romana-Eguia

  3. Laboratory of Integrative Aquatic Biology, Onagawa Field Center, Graduate School of Agricultural Science, Onagawa, Oshika, Miyagi, 986-2242, Japan

    Minoru Ikeda

Authors
  1. Brian S. Santos
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  2. Maria Rowena R. Romana-Eguia
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  3. Zubaida U. Basiao
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  4. Minoru Ikeda
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Corresponding author

Correspondence to Brian S. Santos.

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Santos, B.S., Romana-Eguia, M.R.R., Basiao, Z.U. et al. Development and characterization of nine novel microsatellite markers for the milkfish Chanos chanos . Conservation Genet Resour 7, 451–453 (2015). https://doi.org/10.1007/s12686-014-0393-3

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  • DOI: https://doi.org/10.1007/s12686-014-0393-3

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