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Isolation and characterization of twelve microsatellite loci of Leiocassis albomargintus (Rendhal) in the Yangtze River

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Abstract

Leiocassis albomargintus (Rendhal) is endemic to the Yangtze River basin in China. By using the fast isolation by amplified fragment length polymorphism of sequences containing repeats protocol, we identified twelve microsatellite loci in a wild population. The number of alleles per locus ranged from 4 to 13. The observed and expected heterozygosity ranged from 0.4887 to 1.000 and from 0.5025 to 0.9180, respectively. Cross-amplification was tested in related species L. tenuifurcatus and L. crassilabris.

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References

  • Ding RH (1994) The fishes of Sichuan province. Sichuan Science & Technology Press, Chengdu

    Google Scholar 

  • Li Q, Wan J (2005) SSRHunter: development of a local searching software for SSR sites. Hereditas 5:808–810 (in Chinese with English abstract)

    Google Scholar 

  • Ma H, He X (2004) Age and growth of Rhinogobio cylindricus in the mainstream of Yangtze River. Chin J Zool 39:55–59 (in Chinese with English abstract)

    Google Scholar 

  • World Commission on Dams (WCD) (2000) Dams and development: a new framework for decision-making. Earthscan, London

    Google Scholar 

  • Yeh FC, Yang R, Boyle TJ, Ye Z, Xiyan JM (2000) POPGENE 32: Microsoft Windows-based freeware for population genetic analysis. 1.32. Molecular Biology and Biotechnology Centre, University of Alberta, Edmonton

  • Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was supported by the Research Project of China Three Gorges Corporation and the Program of Fisheries Genetic Resources Conservation from Ministry of Agriculture of the P. R. China (2014) to Y. Li.

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Authors and Affiliations

  1. College of Animal Science and Technology, Southwest University, Chongqing, 400716, China

    Jiawen Ba & Yun Li

  2. Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Wuhan, 430223, China

    Jiawen Ba, Shuhua Li, Dengqiang Wang, Xinbin Duan & Daqing Chen

Authors
  1. Jiawen Ba
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  2. Shuhua Li
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  3. Yun Li
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  4. Dengqiang Wang
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  5. Xinbin Duan
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  6. Daqing Chen
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Corresponding author

Correspondence to Yun Li.

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Ba, J., Li, S., Li, Y. et al. Isolation and characterization of twelve microsatellite loci of Leiocassis albomargintus (Rendhal) in the Yangtze River. Conservation Genet Resour 7, 315–317 (2015). https://doi.org/10.1007/s12686-014-0374-6

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  • DOI: https://doi.org/10.1007/s12686-014-0374-6

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