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Differentiation of white-footed mice (Peromyscus leucopus) and deer mice (Peromyscus maniculatus) of the Upper Midwest using PCR melt curve analysis

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Abstract

Cryptic species pairs including white-footed mice, Peromyscus leucopus, and deer mice, Peromyscus maniculatus, are difficult to identify using morphological and laboratory techniques. To improve rapid and accurate determination of these species, we developed an assay reliant on differences in PCR-product melt curves (MCA). We differentiated 235 P. maniculatus gracilis and 78 P. leucopus using our assay. We compared this to morphological identification using ear length, which also provided reliable species identification. We find that MCA accurately differentiates these species and provides more reliable identifications than certain morphological measures, therefore making our approach ideal for identification of these species.

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References

  • Aquadro C, Patton J (1980) Salivary amylase variation in Peromyscus—Use in species identification. J Mammal 61:703–707. doi:10.2307/1380315

    Article  Google Scholar 

  • Bickford D, Lohman DJ, Sodhi NS, Ng PKL, Meier R, Winker K, Ingram KK, Das I (2007) Cryptic species as a window on diversity and conservation. Trends Ecol Evol 22:148–155. doi:10.1016/j.tree.2006.11.004

    Article  PubMed  Google Scholar 

  • Evangelou VI, Bouga M, Emmanouel NG, Perdikis DC, Papadoulis GT. (2013) Discrimination of two natural biocontrol agents in the mediterranean region based on mitochondrial DNA sequencing data. Biochem Genetics 51. doi:10.1007/s10528-013-9610-0

  • Kamler JF, Pennock DS, Welch C, Pierotti RJ (1998) Variation in morphological characteristics of the white-footed mouse (Peromyscus leucopus) and the deer mouse (P. maniculatus) under allotopic and syntopic conditions. Am Midl Nat 140:170–179. doi:10.1674/0003-0031(1998)140[0170:VIMCOT]2.0.CO;2

  • Lalonde LF, Reyes J, Gajadhar AA. (2013) Application of a qPCR assay with melting curve analysis for detection and differentiation of protozoan oocysts in human fecal samples from Dominican Republic. Am J Trop Med Hyg 89. doi:10.4269/ajtmh.13-0106

  • Lindquist E, Aquadro C, McClearn D, McGowan K (2003) Field identification of the mice Peromyscus leucopus noveboracensis and P. maniculatus gracilis in central New York. Can Field-Nat 117:184–189

    Google Scholar 

  • Myers P, Lundrigan BL, Hoffman SMG, Haraminac AP, Seto SH (2009) Climate-induced changes in the small mammal communities of the Northern Great Lakes Region. Global Change Biol 15:1434–1454

    Article  Google Scholar 

  • Rich S, Kilpatrick C, Shippee J, Crowell K (1996) Morphological differentiation and identification of Peromyscus leucopus and P. maniculatus in northeastern North America. J Mammal 77:985–991. doi:10.2307/1382779

    Article  Google Scholar 

  • Sattler T, Bontadina F, Hirzel AH, Arlettaz R (2007) Ecological niche modelling of two cryptic bat species calls for a reassessment of their conservation status. J Appl Ecol 44:1188–1199. doi:10.1111/j.1365-2664.2007.01328.x

    Article  Google Scholar 

  • Sikes RS, Gannon WL, Animal Care and Use Committee of the American Society of Mammalogists (2011) Guidelines of the American Society of Mammalogists for the use of wild mammals in research. J Mammal 92:235–253. doi:10.1644/10-MAMM-F-355.1

    Article  Google Scholar 

  • Stephens RB, Anderson EM, Wendt SR, Meece JK (2014) Field identification of sympatric Peromyscus leucopus noveboracensis and P. maniculatus gracilis in Wisconsin from external measurements. Am Midl Nat 171:139–146

    Article  Google Scholar 

  • Sternburg J, Feldhamer G (1997) Mensural discrimination between sympatric Peromyscus leucopus and P. maniculatus in southern Illinois. Acta Therio 42:1–13

    Article  Google Scholar 

Download references

Acknowledgments

The Eck Institute for Global Health and UNDERC supported this research. Andrew Bantel and Mary Oswald assisted in laboratory procedures. Samantha Driscoll, Lauren Eckert, Chelsea Gyure, and Matt Igleski helped collect samples.

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  1. B. J. Ridenhour

    Present address: Department of Biological Sciences, University of Idaho, Moscow, ID, 83844, USA

Authors and Affiliations

  1. Department of Biological Sciences, Eck Institute for Global Health, University of Notre Dame, Notre Dame, IN, 46556, USA

    B. J. Ridenhour

  2. Department of Biological Sciences, University of Notre Dame Environmental Research Center, University of Notre Dame, 97 Galvin Life Sciences Bldg, Notre Dame, IN, 46556, USA

    M. J. Cramer

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  1. B. J. Ridenhour
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  2. M. J. Cramer
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Correspondence to M. J. Cramer.

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Ridenhour, B.J., Cramer, M.J. Differentiation of white-footed mice (Peromyscus leucopus) and deer mice (Peromyscus maniculatus) of the Upper Midwest using PCR melt curve analysis. Conservation Genet Resour 7, 29–31 (2015). https://doi.org/10.1007/s12686-014-0324-3

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  • DOI: https://doi.org/10.1007/s12686-014-0324-3

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