ABSTRACT
The Fiscal Flycatcher, Sigelus silens, is the only representative of a monotypic genus, endemic to Southern Africa, and may represent two cryptic species. Here we describe the development of thirteen microsatellite markers, and characterize polymorphism for each one. We found that all but one of our 13 loci were highly variable, each having five or more alleles. This suggests that these markers will have high variability across the species range and will be of utility in understanding the extent of gene flow among populations.
References
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods in Enzymol 395:202–222
Martins WS, Lucas DCS, Neves KFS, Bertioli DJ (2009) WebSat- a web software for microsatellite marker development. Bioinformation 3:282–283
Paquette S (2013) PopGenKit: Useful functions for (batch) file conversion and data resampling in microsatellite datasets. CRAN
Paradis E (2010) pegas: an R package for population genetics with an integrated-modular approach. Bioinformatics 26:419–420
van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Acknowledgments
The authors thank Hanneline Smit, Graeme Oatley, Ângela Ribeiro, Dawie de Swardt and Jérôme Fuchs for help with collecting samples. This research was supported by NSF grants to R.C.K.B and G.V. (DEB1120356, DEB1119931). This is publication 1478 of the Biodiversity Research and Teaching Collections at Texas A&M.
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Wogan, G.O.U., Feldheim, K.A., Voelker, G. et al. Development and characterization of thirteen microsatellite markers for the Fiscal Flycatcher (Sigelus silens) for use in phylogeographic and landscape genetics research. Conservation Genet Resour 7, 125–127 (2015). https://doi.org/10.1007/s12686-014-0309-2
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DOI: https://doi.org/10.1007/s12686-014-0309-2