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Characterisation of nine European wall lizard (Podarcis muralis) microsatellite loci of utility across sub-species

  • Microsatellite Letters
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Abstract

Human-assisted dispersal of the European wall lizard (Podarcis muralis) has created conservation concerns due to extensive introgression between normally allopatric and genetically distinct lineages. We characterised nine microsatellite loci that amplified in four mtDNA clades belonging to two main evolutionary lineages (broadly belonging to P. m. brogniardii and P. m. nigriventris) known to hybridise in England and Germany. All loci were autosomal and displayed 5–28 alleles. These loci will prove useful for population genetic and parentage studies in hybrid zones.

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Acknowledgments

This work was supported by the UK Natural Environment Research Council (NERC). We thank Gavin Horsburgh for preparing the repeat-enriched library, Christian Bourne, John Kenny and Xuan Liu for 454 pyrosequencing, and Terry Burke for helpful comments on the manuscript. Microsatellite isolation and genotyping was performed at the NERC Biomolecular Analysis Facility (NBAF) in Sheffield and pyrosequencing at the NBAF–Liverpool, UK. TU is supported by the Royal Society of London.

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Author notes

  1. Robert J. P. Heathcote

    Present address: Centre for Research in Animal Behaviour, College of Life and Environmental Sciences, University of Exeter, Perry Road, Exeter, EX4 4QJ, UK

Authors and Affiliations

  1. Edward Grey Institute, Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK

    Robert J. P. Heathcote & Tobias Uller

  2. Department of Animal and Plant Sciences, University of Sheffield, Sheffield, S10 2TN, UK

    Robert J. P. Heathcote & Deborah A. Dawson

Authors
  1. Robert J. P. Heathcote
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  2. Deborah A. Dawson
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  3. Tobias Uller
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Corresponding author

Correspondence to Robert J. P. Heathcote.

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Heathcote, R.J.P., Dawson, D.A. & Uller, T. Characterisation of nine European wall lizard (Podarcis muralis) microsatellite loci of utility across sub-species. Conservation Genet Resour 7, 85–87 (2015). https://doi.org/10.1007/s12686-014-0294-5

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  • DOI: https://doi.org/10.1007/s12686-014-0294-5

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