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Development and characterization of 38 microsatellite markers for Camellia flavida based on transcriptome sequencing

  • Microsatellite Letters
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Abstract

Camellia flavida is an endangered and valuable ornamental plant species with yellow flower. Here we isolated and characterized 38 polymorphic microsatellite loci for C. flavida using transcriptome sequencing. The markers were tested on one population of C. flavida and one population of closely related C. nitidissima. For C. fladviia, all of these loci showed polymorphism, and the number of alleles per locus ranged from 2 to 8. The observed and expected heterozygosities ranged from 0.250 to 0.900 and from 0.250 to 0.789, respectively. These markers will be useful in the research on the genetic diversity and population genetic structure of C. flavida. These loci can also be used for other related Camellia species.

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Acknowledgments

The work was supported by the National Natural Science Foundation of China (31260053) and Project of Graduate Base Construction of Guangxi.

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Authors and Affiliations

  1. Ministry of Education Key Laboratory for Ecology of Rare and Endangered Species and Environmental Protection, School of Life Sciences, Guangxi Normal University, Guilin, 541004, China

    Yong-Qing Liufu, Guo-Qing Peng, Yong-Bin Lu, Quan-Qing Ye & Shao-Qing Tang

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  1. Yong-Qing Liufu
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  2. Guo-Qing Peng
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  3. Yong-Bin Lu
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  4. Quan-Qing Ye
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  5. Shao-Qing Tang
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Corresponding author

Correspondence to Shao-Qing Tang.

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Liufu, YQ., Peng, GQ., Lu, YB. et al. Development and characterization of 38 microsatellite markers for Camellia flavida based on transcriptome sequencing. Conservation Genet Resour 6, 1007–1010 (2014). https://doi.org/10.1007/s12686-014-0270-0

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  • DOI: https://doi.org/10.1007/s12686-014-0270-0

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