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Characterisation of 9 polymorphic microsatellite markers for the Critically Endangered lemur leaf frog Agalychnis lemur

  • Microsatellite Letters
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Abstract

We characterise nine polymorphic microsatellites for the Critically Endangered lemur leaf frog, Agalychnis lemur. We found between 3 and 8 alleles per locus in 48 captive individuals originating from the last two currently known remaining wild populations of these frogs in Costa Rica. We attribute observed deviations from Hardy–Weinberg and linkage equilibrium to inbreeding and population substructure in captivity. The newly designed loci will be used to establish the first genetically-informed amphibian studbook for captive breeding and conservation purposes.

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References

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Acknowledgments

We are grateful to Adam Bland of Manchester Museum, for his help and assistance with the rearing and sampling of captive specimens. Samples in Costa Rica were collected and exported with special permission from Costa Rican authorities: MINAE (collection/export permit: Resolution no: 255-2000-OFAU). We would especially like to thank Snr Javier Guevara for all his support.

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Authors and Affiliations

  1. School of Environment and Life Sciences, University of Salford, Salford, M5 4WT, UK

    Alex Petchey & Robert Jehle

  2. Manchester Museum, University of Manchester, Oxford Road, Manchester, M13 9PL, UK

    Andrew Gray

  3. Nordens Ark, Åby säteri, SE-456 93, Hunnebostrand, Sweden

    Claes Andrén

  4. Bristol Zoological Society, Bristol Zoo Gardens, Clifton, Bristol, BS8 3HA, UK

    Tim Skelton

  5. Costa Rican Amphibian Centre, Guyacan, Costa Rica

    Brian Kubicki

  6. Berkshire College of Agriculture, Burchetts Green, Maidenhead, Berkshire, SL6 6QR, UK

    Calvin Allen

Authors
  1. Alex Petchey
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  2. Andrew Gray
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  3. Claes Andrén
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  4. Tim Skelton
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  5. Brian Kubicki
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  6. Calvin Allen
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  7. Robert Jehle
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Corresponding author

Correspondence to Alex Petchey.

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Petchey, A., Gray, A., Andrén, C. et al. Characterisation of 9 polymorphic microsatellite markers for the Critically Endangered lemur leaf frog Agalychnis lemur . Conservation Genet Resour 6, 971–973 (2014). https://doi.org/10.1007/s12686-014-0261-1

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  • DOI: https://doi.org/10.1007/s12686-014-0261-1

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