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Isolation and characterization of 42 microsatellite loci from the Hemifusus tuba Gmelin

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Abstract

Hemifusus tuba Gmelin is a commercially important gastropod species, but has been suffering from severe population decline due to overexploitation and the damage of natural habitat. In this study, 42 microsatellite DNA markers were developed from transcriptome sequences (alleles/locus: 2–10; observed heterozygosity: 0.067–1.000, expected heterozygosity: 0.097–0.897). Five loci were found deviate significantly from Hardy–Weinberg equilibrium. These microsatellite loci will contribute to the effective genetic conservation management of this species.

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Acknowledgments

This study was supported by the Grants from National Natural Science Foundation of China (No. 41206113), the Scientific and Technical Supporting Program (2011BAD13B0903), Educational Commission of Zhejiang Province (No. Y201224623), Natural Science Foundation of Ningbo (No. 2012A610139), Key Discipline Program of Ningbo (No. XKL11D2101) and K C Wong Magana Fund in Ningbo University.

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Correspondence to Ronghua Li.

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Wu, L., Li, R., Wang, C. et al. Isolation and characterization of 42 microsatellite loci from the Hemifusus tuba Gmelin. Conservation Genet Resour 6, 707–710 (2014). https://doi.org/10.1007/s12686-014-0193-9

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  • DOI: https://doi.org/10.1007/s12686-014-0193-9

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