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Characterisation of 15 novel microsatellite loci for the grey reef shark (Carcharhinus amblyrhynchos)

  • Microsatellite Letters
  • Published:
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Abstract

Grey reef sharks (Carcharhinus amblyrhynchos) are important apex predators on coral reefs, and their numbers have declined dramatically as a result of overfishing. Knowledge of environmental factors that shape gene flow is essential for developing appropriate management strategies, but the lack of suitable genetic markers has hindered research on this species. Here, we characterised 15 polymorphic microsatellite loci for grey reef sharks. None of the loci deviated significantly from Hardy–Weinberg equilibrium and there was no evidence of Linkage Disequilibrium. Several loci cross-amplified in other carcharhinid species, and will be useful in future studies of this family.

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Acknowledgments

This study was funded by the Department of Biological Sciences at Macquarie University and the Sea World Research and Rescue Foundation. We would like to thank Siobhan Dennison and Nicolette Armansin for laboratory assistance.

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Authors and Affiliations

  1. Conservation Genetics Lab, Department of Biological Sciences, Macquarie University, North Ryde, NSW, 2109, Australia

    Paolo Momigliano & Adam Stow

  2. Sydney Institute of Marine Science, Mosman, NSW, 2089, Australia

    Paolo Momigliano

  3. Wildlife Marine, Perth, WA, 6020, Australia

    William D. Robbins

  4. School of Marine and Tropical Biology, James Cook University, Townsville, QLD, 4814, Australia

    William D. Robbins

  5. School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA, 5001, Australia

    Michael Gardner

  6. Australian Centre for Evolutionary Biology and Biodiversity, University of Adelaide, Adelaide, SA, 5005, Australia

    Michael Gardner

  7. Evolutionary Biology Unit, South Australian Museum, North Terrace Adelaide, SA, 5000, Australia

    Michael Gardner

Authors
  1. Paolo Momigliano
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  2. William D. Robbins
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  3. Michael Gardner
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  4. Adam Stow
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Corresponding author

Correspondence to Paolo Momigliano.

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Momigliano, P., Robbins, W.D., Gardner, M. et al. Characterisation of 15 novel microsatellite loci for the grey reef shark (Carcharhinus amblyrhynchos). Conservation Genet Resour 6, 661–663 (2014). https://doi.org/10.1007/s12686-014-0174-z

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  • DOI: https://doi.org/10.1007/s12686-014-0174-z

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