Conservation Genetics Resources

, Volume 6, Issue 3, pp 531–533

Development of 24 tetra-nucleotide microsatellite markers in Cortes Geoduck Panopea globosa by next-generation sequencing

  • Pedro Cruz-Hernández
  • Adrian Munguia-Vega
  • Ignacio Leyva-Valencia
  • Fátima Lucero-Burquez
  • Daniel B. Lluch-Cota
Technical Note

DOI: 10.1007/s12686-014-0172-1

Cite this article as:
Cruz-Hernández, P., Munguia-Vega, A., Leyva-Valencia, I. et al. Conservation Genet Resour (2014) 6: 531. doi:10.1007/s12686-014-0172-1

Abstract

Panopea globosa is a marine bivalve mollusk important for small-scale fisheries from Northwest Mexico. Twenty-four tetra-nucleotide microsatellite markers were characterized by 454 XL+ Titanium pyrosequencing. High average levels of allelic diversity and heterozygosity were observed (NA = 16.68; Ne = 9.77; HO = 0.643; HE = 0.858). Significant deviations from HWE, as previously observed in other mollusks, were detected in twelve loci. Linkage disequilibrium was detected among two loci pairs. This set of microsatellite loci will be useful to determine the genetic structure and larval connectivity of P. globosa.

Keywords

Tetra-nucleotide microsatellite Panopea Gulf of California Marine connectivity Next generation sequencing 

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • Pedro Cruz-Hernández
    • 1
  • Adrian Munguia-Vega
    • 2
    • 3
  • Ignacio Leyva-Valencia
    • 1
  • Fátima Lucero-Burquez
    • 1
  • Daniel B. Lluch-Cota
    • 1
  1. 1.Centro de Investigaciones Biológicas del Noroeste, S.C.Instituto Politécnico NacionalLa PazMexico
  2. 2.PANGAS Science CoordinationComunidad y Biodiversidad A.C.GuaymasMexico
  3. 3.Conservation Genetics Laboratory, School of Natural Resources and the EnvironmentUniversity of ArizonaTucsonUSA