Abstract
Fourteen microsatellite loci were isolated from Mesogyne insignis, a threatened, African understory tree. Alleles ranged between two and eight per locus, with expected heterozygosity ranging from 0.063 to 0.845 and observed heterozygosity ranging from 0.000 to 0.733. One locus departed from Hardy–Weinberg equilibrium leaving 13 polymorphic loci that will be used to study the genetic variability of fragmented populations so as to enhance the species conservation efforts.
This is a preview of subscription content, log in via an institution to check access.
References
Berg CC (1977) Revisions of African Moraceae (excluding Dorstenia, Ficus, Musanga and Myrianthus) Bull du Jard Bot Natl de Belg 47:267–407
Brooks TM, Mittermeier RA, Mittermeier CG, da Fonseca GAB, Rylands AB, Konstant WR, Flick P, Pilgrim P, Oldfield S, Magin G, Hilton-Taylor C (2002) Habitat loss and extinction in the hotspots of biodiversity. Conserv Biol 16:909–923
Datwyler SL, Weiblen GD (2004) On the origin of the fig: phylogenetic relationships of Moraceae from ndhF sequences. Am J Bot 91:767–777
Figueiredo E (1994) Diversity and endemism of angiosperms in the Gulf of Guinea islands. Biodivers Conserv 3:785–793
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
IUCN (2012) Mesogyne insignis. In: IUCN red list of threatened species. Version 2012.1. www.iucnredlist.org. Downloaded on 16 August 2012
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655
Raymond ML, Rousset F (1995) An exact test for population differentiation. Evol 49:1280–1283
Rice WR (1989) Analyzing tables of statistical tests. Evol 43:223–225
Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234
Acknowledgments
We developed microsatellites in the Pritzker Laboratory for Molecular Systematics and Evolution operated with support from the Pritzker Foundation. We thank Amani Nature Reserve, Tanzania Commission for Science and Technology (COSTECH), University of Dar es Salaam, Field Museum and Roosevelt University for support.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Murdoch, K.C., Ndangalasi, H.J., LeCaptain, M.K. et al. Isolation and development of 13 new, polymorphic microsatellite loci for a threatened, understory tree, Mesogyne insignis, (Moraceae) from the Eastern Arc Mountains. Conservation Genet Resour 5, 275–277 (2013). https://doi.org/10.1007/s12686-012-9786-3
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12686-012-9786-3