Conservation Genetics Resources

, Volume 4, Issue 2, pp 343–345

Novel tetra- and pentanucleotide microsatellite markers allow for multiplexed genotyping of Sulawesi tarsiers (Tarsius spp.)

  • Stefan Merker
  • Désirée Boucsein
  • Barbara Feldmeyer
  • Dyah Perwitasari-Farajallah
  • Bruno Streit
Technical Note

DOI: 10.1007/s12686-011-9543-z

Cite this article as:
Merker, S., Boucsein, D., Feldmeyer, B. et al. Conservation Genet Resour (2012) 4: 343. doi:10.1007/s12686-011-9543-z
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Abstract

Based on 454 sequencing, we characterized 10 polymorphic tetra- and pentanucleotide microsatellites for population studies of Sulawesi tarsiers. We identified 2516 repeat regions and designed PCR primer pairs for 60 tetra-, penta- and hexanucleotide repeat loci. Of 16 markers tested with dye-labelled primers, 11 proved to be polymorphic and 10 were readily amplified in multiplex PCR reactions. We tested 54 individuals of three species (T. lariang, T. dentatus, T. wallacei) and 10 putative hybrids for allelic diversity. All loci are genetically unlinked, one locus was found to deviate from Hardy–Weinberg equilibrium in one species. The novel marker system will aid studies of tarsier landscape genetics in the biodiversity hotspot Sulawesi.

Keywords

454 Sequencing Microsatellites Primates Sulawesi Tarsius 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Stefan Merker
    • 1
  • Désirée Boucsein
    • 1
  • Barbara Feldmeyer
    • 2
  • Dyah Perwitasari-Farajallah
    • 3
    • 4
  • Bruno Streit
    • 1
  1. 1.Evolutionary Ecology GroupGoethe University FrankfurtFrankfurt am MainGermany
  2. 2.Molecular Ecology GroupForschungszentrum Biodiversität und KlimaFrankfurt am MainGermany
  3. 3.Primate Research CenterBogor Agricultural UniversityBogorIndonesia
  4. 4.Department of BiologyBogor Agricultural UniversityBogorIndonesia

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