Conservation Genetics Resources

, Volume 4, Issue 2, pp 265–268

New tetranucleotide microsatellite loci in pink abalone (Haliotis corrugata) isolated via 454 pyrosequencing

  • A. P. Greenley
  • A. Muguia-Vega
  • A. Saenz-Arroyo
  • F. Micheli
Technical Note

DOI: 10.1007/s12686-011-9521-5

Cite this article as:
Greenley, A.P., Muguia-Vega, A., Saenz-Arroyo, A. et al. Conservation Genet Resour (2012) 4: 265. doi:10.1007/s12686-011-9521-5

Abstract

Microsatellite loci were developed for the pink abalone (Haliotis corrugata) via 454 high-throughput sequencing. From 193 tetranucleotide repeats identified from 77.5 Mb of sequence, we tested 80 loci and successfully amplified and scored 18 microsatellite markers. All loci were polymorphic with number of alleles ranging from 5 to 21. Average observed and expected heterozygosities were 0.745 and 0.844, respectively. Three loci deviated from Hardy–Weinberg equilibrium, two of which had significant deficits of heterozygotes and only one displayed statistical evidence of a null allele. None of the loci exhibited linkage disequilibrium. These loci are a valuable asset for fine-scale population genetic and paternity studies centered on the conservation and management of pink abalone.

Keywords

Baja California Fisheries Haliotis corrugata Abalone Microsatellites Paternity 

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • A. P. Greenley
    • 1
  • A. Muguia-Vega
    • 2
  • A. Saenz-Arroyo
    • 3
  • F. Micheli
    • 1
  1. 1.Hopkins Marine StationStanford UniversityPacific GroveUSA
  2. 2.Conservation Genetics Laboratory, School of Natural Resources and the EnvironmentThe University of ArizonaTucsonUSA
  3. 3.Comunidad y Biodiversidad A.C.GuaymasMexico

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