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Twelve novel microsatellite markers for the marbled salamander, Ambystoma opacum

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Abstract

We isolated and characterized a total of 12 microsatellite loci from the marbled salamander, Ambystoma opacum. Loci were screened in 24 individuals from a single location on the Savannah River Site in South Carolina. The number of alleles per locus ranged from 3 to 15, observed heterozygosity ranged from 0.167 to 0.917, and the probability of identity values ranged from 8.5 × 10−2 to 3.2 × 10−1. These new loci will provide tools for examining the genetic diversity, structure, and mating system of A. opacum populations.

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References

  • Bartoszek J, Greenwald KR (2009) A population divided: railroad tracks as barriers to gene flow in an isolated population of marbled salamanders (Ambystoma opacum). Herpetol Conserv Biol 4:191–197

    Google Scholar 

  • Croshaw D, Schable N, Peters MB, Glenn TC (2005) Isolation and characterization of microsatellite DNA loci from Ambystoma salamanders. Conserv Genet 6:473–479

    Article  Google Scholar 

  • DeWoody AJ, Schupp J, Kenefic L et al (2004) Universal method for producing ROX-labeled size standards suitable for automated genotyping. BioTechniques 37:348–350

    PubMed  CAS  Google Scholar 

  • Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991) ‘Touchdown’ PCR to circumvent spurious priming during gene amplification. Nucleic Acids Res 19:4008

    Article  PubMed  CAS  Google Scholar 

  • Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222

    Article  PubMed  CAS  Google Scholar 

  • Greenwald KR, Gibbs HL, Waite T (2009) Efficacy of land-cover models in predicting isolation of marbled salamander populations in a fragmented landscape. Conserv Biol 23:1232–1241

    Article  PubMed  Google Scholar 

  • Lance SL, Light JE, Jones KL, Hagen C, Hafner JC (2010) Isolation and characterization of 17 polymorphic microsatellite loci in the kangaroo mouse, genus Microdipodops (Rodentia: Heteromyidae). Conserv Genet Resour 2:139–141. doi:10.1007/s12686-010-9195-4

    Article  Google Scholar 

  • Peakall R, Smouse PE (2006) GENALEX 6: genetic analysis in excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295

    Article  Google Scholar 

  • Petranka JW (1998) Salamanders of the United States and Canada. Smithsonian Institution Press, Washington

    Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Res 8:103–106

    Article  Google Scholar 

  • Scott DE (2005) Ambystoma opacum, Marbled Salamander. In: Lannoo MJ (ed) Amphibian declines: the conservation status of United States species. University of California Press, Berkeley

    Google Scholar 

  • Snodgrass JW, Komoroski MJ, Bryan AL, Burger J (2000) Relationaships among isolated wetland size, hydroperiod, and amphibian species richness: implications for wetland regulations. Con Biol 14:414–419

    Article  Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Willis PM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references

Acknowledgments

Manuscript preparation was partially supported by the DOE under Award Number DE-FC09-07SR22506 to the University of Georgia Research Foundation.

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Authors and Affiliations

  1. Savannah River Ecology Laboratory, University of Georgia, Drawer E, Aiken, SC, 29802, USA

    Schyler O. Nunziata, David E. Scott, Cris Hagen & Stacey L. Lance

  2. Department of Biological Sciences, Eastern Kentucky University, Richmond, KY, 40475, USA

    Schyler O. Nunziata

  3. Department of Biochemistry and Molecular Genetics, School of Medicine, University of Colorado, Aurora, CO, 80045, USA

    Kenneth L. Jones

Authors
  1. Schyler O. Nunziata
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  2. David E. Scott
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  3. Kenneth L. Jones
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  4. Cris Hagen
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  5. Stacey L. Lance
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Correspondence to Stacey L. Lance.

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This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof.

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Nunziata, S.O., Scott, D.E., Jones, K.L. et al. Twelve novel microsatellite markers for the marbled salamander, Ambystoma opacum . Conservation Genet Resour 3, 773–775 (2011). https://doi.org/10.1007/s12686-011-9455-y

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  • DOI: https://doi.org/10.1007/s12686-011-9455-y

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