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New microsatellite markers for the assessment of fine-scale dispersal patterns in the endangered montane caddisfly Drusus discolor

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Abstract

We present a panel of variable microsatellite markers for the montane caddisfly Drusus discolor, a rare species that we use as a model for the assessment of climate change effects on the montane freshwater community. 454 sequencing technology was used in order to generate random sequence data. Of a total of 2,648 microsatellite-carrying fragments identified, 11 variable, reproducible markers were generated and tested on 41 individuals from two populations. Allelic diversity ranged from 3 to 10 alleles and heterozygosity values varied from 0 to 0.89 (H O) and 0.12 to 0.86 (H E). A cross-species test on four caddisfly species showed that a subset of markers amplifies in related taxa.

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Acknowledgments

We thank Steffen U. Pauls (Biodiversity and Climate Research Center) for providing samples for the cross-species test. Funding comes from the Deutsche Forschungsgemeinschaft (HA3431/4-1). Additional funding is provided by the research funding program Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz (LOEWE) of Hesse’s Ministry of Higher Education, Research, and the Arts.

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Authors and Affiliations

  1. Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, 60325, Frankfurt am Main, Germany

    Jutta Geismar, Jan Sauer, Peter Haase & Carsten Nowak

  2. Department of Limnology and Conservation, Senckenberg Research Institutes and Natural History Museums, Clamecystrasse 12, Gelnhausen, Germany

    Jutta Geismar, Peter Haase & Carsten Nowak

Authors
  1. Jutta Geismar
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  2. Jan Sauer
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  3. Peter Haase
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  4. Carsten Nowak
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Correspondence to Carsten Nowak.

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Geismar, J., Sauer, J., Haase, P. et al. New microsatellite markers for the assessment of fine-scale dispersal patterns in the endangered montane caddisfly Drusus discolor . Conservation Genet Resour 3, 605–607 (2011). https://doi.org/10.1007/s12686-011-9414-7

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  • DOI: https://doi.org/10.1007/s12686-011-9414-7

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